Distribution and importance of extracellular DNA in the subsurface sediments

2009 Fiscal Year Final Research Report

• Project/Area Number: 19740315
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Geology
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: TAKEUCHI Mio National Institute of Advanced Industrial Science and Technology, 地圏資源環境研究部門, 主任研究員 (20357403)
• Project Period (FY): 2007 – 2009
• Keywords: 細胞外DNA / 地下圏微生物 / RNA / 古細菌 / 微生物相

Research Abstract

Extracellular DNA was quantified with terrestrial subsurface sediments. Extracellular DNA was detected at 2.9-28.3 ng/g dry weight sediment and decreased with depth. Microbial community analysis using the extracellular DNA yielded significantly low number of OTUs, suggesting that the extracellular DNA was fragmented or damaged in the environment. RNA based community analysis revealed variety of active microbes (Proteobacteria, Chloroflexi, Acidobacteria, Miscellaneous Crenarchaeotic Group (MCG), ANME) in the terrestrial subsurface environment.

Research Products

• [Journal Article] Bacterial and Archaeal 16S rRNA Genes in Late Pleistocene to Holocene Muddy Sediments from the Kanto Plain of Japan (2009)
  • Author(s): Takeuchi, M., Komai, T., Hanada, S., Tamaki, H., Tanabe, S., Miyachi, Y., Uchiyama, M., Nakazawa, T., Kimura, K., Kamagata, Y.
  • Journal Title: Geomicrobiology Journal 26:2 Pages: 104-118
  • Peer Reviewed
• [Journal Article] 地下圏微生物活動に対する難透水層の役割 (2007)
  • Author(s): 竹内美緒
  • Journal Title: 地学雑誌 vol.116,no.6 Pages: 915-921
  • Peer Reviewed
• [Presentation] Microbial world in the subsurface of Tokyo (2009)
  • Author(s): Takeuchi M.
  • Organizer: 1st Korea-Japan symposium
  • Place of Presentation: Cheju island, Korea
  • Year and Date: 20090000
• [Presentation] 関東平野の沖積粘土層中におけるメタン生成 (2008)
  • Author(s): 竹内美緒, 玉木秀幸, 田邉晋, 内山美 恵子, 高橋学, 吉岡秀佳
  • Organizer: アーキア研究会
  • Place of Presentation: 那覇, 沖縄
  • Year and Date: 20080000
• [Presentation] Living microbial community (methanogens) in ancient marine sediments of Holocene in the Kanto Plain (2008)
  • Author(s): M. Takeuchi, H. Tamaki, S. Tanabe, M. Uchiyama, M. Sugihara, M. Takahashi, H. Yoshioka
  • Organizer: Japan, ISSM
  • Place of Presentation: Shizuoka
  • Year and Date: 20080000
• [Presentation] Living methanogens in the Holocene mud (2007)
  • Author(s): M. Takeuchi, H, Tamaki, S, Tanabe, M, Uchiyama, M, Takahashi, H, Yoshioka
  • Organizer: ISME-Asia. Matsuyama
  • Place of Presentation: Ehime
  • Year and Date: 20070000