2009 Fiscal Year Final Research Report
Development of a novel system for monitoring cellular ATP using a unique enzyme reaction
| Project/Area Number |
19750063
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
SUEDA Shinji Kyushu Institute of Technology, 大学院・情報工学研究院, 准教授 (00325581)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ATP検出 / ビオチン化酵素 / 蛍光タンパク質 / 蛍光共鳴エネルギー移動 |
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| Research Abstract |
In the biotinylation reaction, biotin protein ligase (BPL) mediates the attachment of biotin to a specific lysine residue of biotin carboxyl carrier protein (BCCP). The biotinylation reaction from thermophilic archaeon Sulfolobus tokodaii has a unique characteristic that the enzyme BPL forms a stable complex with its product, biotinylated BCCP. In the present work, we have developed a detection system for ATP with this unique biotinylation reaction.
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