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2009 Fiscal Year Final Research Report

Design of enzymatic glycosylation reaction system toward unrecognized substrates such as macromolecules and interface.

Research Project

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Project/Area Number 19750086
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Polymer chemistry
Research InstitutionTohoku University

Principal Investigator

KOBAYASHI Atsushi  Tohoku University, 大学院・工学研究科, 助教 (90361138)

Project Period (FY) 2007 – 2009
Keywords糖鎖高分子 / 酵素触媒高分子反応 / アマドリ転位反応
Research Abstract

It is very important to develop the addition reaction onto macromolecules and interfaces by enzymes, although these substrates are difficult to be accepted by the enzymes. In this research, I successfully developed the improved enzymatic glycosylation reaction system by the simple chemical treatment of these substrates.

  • Research Products

    (14 results)

All 2009 2008 2007 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (3 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Efficient Synthesis of Sugar Oxazolines from Unprotected N-Acetyl-2-amino Sugars by Using Chloroformamidinium Reagent in Water.2009

    • Author(s)
      Noguchi, M., Tanaka, T., Gyakushi, H., Kobayashi, A., Shoda, S.
    • Journal Title

      J.Org.Chem. 74

      Pages: 2210-2212

    • Peer Reviewed
  • [Journal Article] One-step conversion of unprotected sugars to β-glycosyl azides using 2-chloroimidazolinium salt in aqueous solution2009

    • Author(s)
      Tanaka, T., Nagai, H., Noguchi, M., Kobayashi, A., Shoda, S.
    • Journal Title

      Chem.Commun.

      Pages: 3378-3379

    • Peer Reviewed
  • [Journal Article] Direct transformation of unprotected sugars to aryl 1-thio-β-glycosides in aqueous media using 2-chloro-1,3-dimethylimidazolinium chloride2009

    • Author(s)
      Tanaka, T., Matsumoto, T., Noguchi, M., Kobayashi, A., Shoda, S.
    • Journal Title

      Chem.Lett. 38

      Pages: 458-459

    • Peer Reviewed
  • [Journal Article] Dimethoxy triazine glycosides as new glycosyl donors for chemo-enzymatic synthesis of oligosaccharides2009

    • Author(s)
      Tanaka, T., Kobayashi, A., Noguchi, M., Kimura, K., Watanabe, K., Shoda, S.
    • Journal Title

      J.Appl.Glycosci. 56

      Pages: 83-88

    • Peer Reviewed
  • [Journal Article] Direct synthesis of 1,6-anhydro sugars from unprotected glycopyranoses by using 2-chloro-1,3-dimethylimidazolinium chloride2009

    • Author(s)
      Tanaka, T., Huang, W., Noguchi, M., Kobayashi, A., Shoda, S.
    • Journal Title

      Tetrahedron Lett. 50

      Pages: 2154-2157

    • Peer Reviewed
  • [Journal Article] Synthesis of artificial oligosaccharides by polycondensation of 2'-O-methyl cellobiosyl fluoride and mannosyl fluoride catalyzed by cellulase, in :J.Kadokawa (Ed.), Interfacial Researches in Fundamental and Material Sciences of Oligo-and Polysaccharides2009

    • Author(s)
      R.Izumi, Y.Suzuki, Y.Shimizu, M.Fujita, M.Ishihara, M.Noguchi, A.Kobayashi, S.Shoda
    • Journal Title

      Transworld Research Network, Kerala

      Pages: 45-68

    • Peer Reviewed
  • [Journal Article] A novel glycosyl donor for chemo-enzymatic oligosaccharide synthesis : 4,6-dimethoxy-1,3,5-triazin-2-yl glycoside.2008

    • Author(s)
      Tanaka, T., Noguchi, M., Kobayashi, A., Shoda, S.
    • Journal Title

      Chem.Commun.

      Pages: 2016-2018

    • Peer Reviewed
  • [Presentation] A new route to labeled oligosaccharides via glycosyl azides in aqueous media.2009

    • Author(s)
      Kobayashi, A., Nagai, H., Tanaka, T., Noguchi, M., Ishihara, M., Shoda, S.
    • Organizer
      20th International Symposium on Glycoconjugates.
    • Place of Presentation
      Puerto Rico, USA
    • Year and Date
      2009-12-03
  • [Presentation] Enzymatic synthesis of glyco-surfactant via ultrasound irradiation onto substrate mixture containing polysaccharide and glycosyl acceptor2008

    • Author(s)
      Kobayashi, A., Yamamoto、K., Itoh, H., Noguchi, M., Shoda, S.
    • Organizer
      24th International Carbohydrate Symposium Oslo
    • Place of Presentation
      Norway
    • Year and Date
      2008-07-29
  • [Presentation] Chemo-enzymatic synthesis of glycoprotein from Hen Egg lysozyme via the Amadori rearrangement2007

    • Author(s)
      Kobayashi, A., Osanai, H, Murakami, T, Noguchi, M., Shoda, S.
    • Organizer
      19th International Symposium on Glycoconjugates Cairns
    • Place of Presentation
      Australia
    • Year and Date
      2007-07-17
  • [Book] 「酵素的グリコシル化に用いる糖供与体の一段階合成技術」複合糖質の化学と最新応用技術2009

    • Author(s)
      野口真人、田中知成、小林厚志、正田晋一郎
    • Total Pages
      83-89
    • Publisher
      シーエムシー出版
  • [Remarks] 2008年度日本農芸化学会東北支部奨励賞受賞「糖質加水分解酵素を駆使する配糖化基盤技術の開発」

  • [Patent(Industrial Property Rights)] 配糖体およびその製造方法2008

    • Inventor(s)
      正田晋一郎、小林厚志、野口真人、田中知成、永井光、松本健
    • Industrial Property Rights Holder
      東北大学
    • Industrial Property Number
      特許,特願2008-150123
    • Filing Date
      2008-06-09
  • [Patent(Industrial Property Rights)] アンヒドロ糖及びその製造方法2007

    • Inventor(s)
      正田晋一郎、小林厚志、野口真人、田中知成
    • Industrial Property Rights Holder
      東北大学
    • Industrial Property Number
      特許,特願2007-254281
    • Filing Date
      2007-09-28

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Published: 2012-02-13   Modified: 2016-04-21  

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