Validation of protein-drug interactions identified by the phage display technology

2008 Fiscal Year Final Research Report

• Project/Area Number: 19750145
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Chemistry related to living body
• Research Institution: Tokyo University of Science
• Principal Investigator: TAKAKUSAGI Yoichi Tokyo University of Science, 理工学部, 助教 (60439916)
• Project Period (FY): 2007 – 2008
• Keywords: 受容体化学

Research Abstract

本研究では、ファージディスプレイ(PD)法による予備試験において同定された医薬小分子の新規相互作用について検証し、それらの結合を確認した。1)抗生物質ロキシスロマイシン(RXM)とRXMのがん血管新生阻害作用の原因タンパク質の候補であるアンジオモチン(Amot)。2)抗腫瘍活性物質カンプトテシン(CPT)の結合候補タンパク質であるヒトプロスタグランジンE2受容体(hEP1)およびスプライシングファクターhnRNP A3。

Research Products

• [Journal Article] Self assembled monolayer (SAM) of small-molecule to efficiently select binding-peptide from phage library using a quartz-crystal microbalance (QCM) device (2009)
  • Author(s): Yoichi Takakusagi, Aiko Suzuki, Fumio Sugawara, Kengo Sakaguchi
  • Journal Title: Proc. of ICCE 17(in press)
  • Peer Reviewed
• [Journal Article] Identification of a methotrexate-binding peptide from a T7 phage display screen using a QCM device (2008)
  • Author(s): Yoichi Takakusagi, Yuki Kuroiwa, Fumio Sugawara, Kengo Sakaguchi
  • Journal Title: Bioorg. Med. Chem 16 Pages: 7410-7414
  • Peer Reviewed
• [Journal Article] Efficient one-cycle affinity selection of binding proteins or peptides specific for a small-molecule using a T7 phage display pool (2008)
  • Author(s): Yoichi Takakusagi, Kouji Kuramochi, Manami Takagi, Tomoe Kusayanagi, Daisuke Manita et al
  • Journal Title: Bioorg. Med. Chem 16 Pages: 9837-9846
  • Peer Reviewed
• [Journal Article] Identification of C10 biotinylated camptothecin (CPT-10-B) binding peptides using T7 phage display screen on a QCM device (2007)
  • Author(s): Yoichi Takakusagi, Kaori Takakusagi, Kouji Kuramochi, Susumu Kobayashi, Fumio Sugawara, Kengo Sakaguchi
  • Journal Title: Bioorg.Med. Chem 15 Pages: 7590-7598
  • Peer Reviewed
• [Presentation] Self-assembled monolayer (SAM) of small-molecule to efficiently select binding-peptide from phage library using a quartz-crystal microbalance (QCM) device (2009)
  • Author(s): Yoichi Takakusagi et al
  • Organizer: ICCE-17
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 20090700
• [Presentation] Camptothecins : Inhibition of the E-prostanoid receptor subtype 1 (EP1) signaling pathways and its'mechanistic aspects (2009)
  • Author(s): Yoichi Takakusagi et al
  • Organizer: The 50th Anniversary Meeting of the American Society of Pharmacognosy
  • Place of Presentation: Honolulu, Hawaii, USA
  • Year and Date: 20090600
• [Presentation] キイロショウジョウバエによる医薬標的分子の評価 (2008)
  • Author(s): 真仁田大輔、高草木洋一他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 20081200
• [Presentation] T7phagdisplay法を用いたロキシスロマイシン結合タンパク質の探索 (2008)
  • Author(s): 鈴木愛こ、高草木洋一他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 20081200
• [Presentation] QCM-PD法で同定した医薬小分子結合ペプチド群からの医薬小分子?標的タンパク質三次元結合様式の推測 (2008)
  • Author(s): 高草木洋一他
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 20081200
• [Presentation] High-throughput and High-accuracy Drug-Target Identifications (2007)
  • Author(s): Fumio Sugawara, Yoichi Takakusagi, Kouji Kuramochi
  • Organizer: The 4th Takeda Science Foundation Symposium on PharmaScience: On the Fronthiers of Chemical Biology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20071200
• [Presentation] Drug-Target Identifications: (1)Affinity Purification of Proteins by Specific Ligand Immobilized on Resins, (2) T7 Phage Display Cloning of Drug-targeting Molecules (2007)
  • Author(s): Fumio Sugawara, Kouji Kuramochi, Yoichi Takakusagi
  • Organizer: BIT's 5th Anniversary Congress of International Drug Discovery Science and Technology (IDDST)
  • Place of Presentation: Shanghai,China
  • Year and Date: 20070500
• [Book] 蛋白質核酸酵素7月号新実験講座 (2009)
  • Author(s): 高草木洋一、高草木香織、菅原二三男、坂口謙吾
  • Total Pages: 7
  • Publisher: 共立出版