Removal of norovirus during drinking water treatment: Application of recombinant virus-like particles

2008 Fiscal Year Final Research Report

• Project/Area Number: 19760368
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: MATSUSHITA Taku Hokkaido University, 大学院・工学研究科, 准教授 (30283401)
• Project Period (FY): 2007 – 2008
• Keywords: ノロウイルス / VLPs / 凝集沈澱処理 / 急速砂ろ過 / 不活化 / 大腸菌ファージ

Research Abstract

遺伝子組換え技術によりノロウイルスVLPs を発現させることに成功した。発現されたVLPs を電子顕微鏡により観察することにより、その形状および大きさ(35.7 ± 3.2 nm)が野生のノロウイルス粒子とほぼ同一であることを確認した。この粒子を用い、凝集(PACl) -沈澱処理における除去性を調べたところ、十分な凝集剤添加条件下(> 1.08 mg-Al/L )において、2 log 程度の除去が可能であることが示された。また、VLPs の除去率は、同時添加した大腸菌ファージQβや大腸菌ファージMS2 に近かった。このことは、凝集沈澱処理では、これらの大腸菌ファージがノロウイルスの指標ウイルスとして使える可能性を示している。本研究では、発現されたノロウイルスVLPs を用いることにより、これまで評価ができていなかったノロウイルスの浄水処理性が評価できることが示された。

Research Products

• [Journal Article] Comparison of behaviors of two surrogates for pathogenic waterborne viruses, bacteriophages Qβ and MS2, during the aluminum coagulation process (2009)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Urasaki, T. and Ohno, K
  • Journal Title: Water Research 43(3) Pages: 605-612
  • Peer Reviewed
• [Journal Article] Comparison of removal performance of two surrogates for pathogenic waterborne viruses, bacteriophage Qβ and MS2, in a coagulation-ceramic microfiltration system (2009)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Kobuke, M. and Ohno, K
  • Journal Title: Journal of Membrane Science 326 Pages: 564-571
  • Peer Reviewed
• [Journal Article] Effects of reversible and irreversible membrane fouling on virus removal by a coagulation-microfiltration system (2008)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y. and Ohno, K
  • Journal Title: Journal of Water Supply: Research and Technology-AQUA 57(7) Pages: 501-506
  • Peer Reviewed
• [Journal Article] Virus removal in a hybrid coagulation-microfiltration system-Investigating mechanisms of virus removal by a combination of PCR and PFU methods (2007)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Ohno, K. and Kobuke, M
  • Journal Title: Water Science and Technology: Water Supply 7(5-6)
  • Peer Reviewed
• [Presentation] Removal of norovirus VLPs in drinking water treatment process (2009)
  • Author(s): Urasaki, T., Matsushita, T., Shirasaki, N., Oshiba, A., Matsui, Y. and Ohno, K
  • Organizer: Proceedings of The 3rd IWA-ASPIRE Conference
  • Place of Presentation: Taipei International Convention Center, Taipei,Taiwan
  • Year and Date: 20091018-22
• [Presentation] Removal of norovirus by a coagulation-ceramic MF hybrid system (2009)
  • Author(s): Matsushita, T., Shirasaki, N., Oshiba, A., Urasaki, T., Matsui, Y. and Ohno, K
  • Organizer: Proceedings of Euromembrane 2009 Conference
  • Place of Presentation: CORUM Conference Centre,Montpellier, France
  • Year and Date: 20090906-10
• [Presentation] Effectiveremoval of virus by ceramic microfiltration with in-line coagulation pretreatment (2009)
  • Author(s): Matsushita, T., Shirasaki, N., Kobuke, M., Matsui, Y. and Ohno, K
  • Organizer: Proceedings of Euromembrane 2009 Conference
  • Place of Presentation: CORUM Conference Centre,Montpellier, France
  • Year and Date: 20090906-10
• [Presentation] Evaluation of norovirus removal performance in a coagulation.ceramic microfiltration process by using recombinant norovirus VLPs (2009)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Urasaki, T., Oshiba, A. and Ohno, K
  • Organizer: Proceedings of 5th IWA Specialised Membnrae Technology Converence for Water and Wastewater Treatment
  • Place of Presentation: Beijing International Convention Center ,Beijing, China
  • Year and Date: 20090901-03
• [Presentation] Application of recombinant norovirus VLPs to evaluate norovirus removal performance in a coagulation.sedimentation.rapid sand filtration process (2009)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Oshiba, A., Urasaki, T. and Ohno, K
  • Organizer: Proceedings of Nanoparticle and Particle Separation 2009
  • Place of Presentation: Duke University Durham, USA,
  • Year and Date: 20090603-05
• [Presentation] 浄水処理におけるノロウイルスの処理性評価 (2009)
  • Author(s): 大芝淳, 白崎伸隆, 浦崎稔史, 松下拓, 松井佳彦, 大野浩一
  • Organizer: 第60回全国水道研究発表会
  • Place of Presentation: 大宮ソニックシティ,さいたま
  • Year and Date: 20090520-22
• [Presentation] ノロウイルス外套タンパク(NV-VLPs) を用いたノロウイルスの浄水処理性評価 (2008)
  • Author(s): 白崎伸隆, 浦崎稔史, 小泓誠, 松下拓, 松井佳彦, 大野浩一
  • Organizer: 第45回環境工学研究フォーラム
  • Place of Presentation: 大阪工業大学, 大阪
  • Year and Date: 20081128-30
• [Presentation] 浄水処理過程におけるバクテリオファージの処理性比較 (2008)
  • Author(s): 浦崎稔史, 白崎伸隆, 小泓誠, 松下拓, 松井佳彦, 大野浩一
  • Organizer: 第16回衛生工学シンポジウム
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 20081113-14
• [Presentation] Coagulation-ceramic microfiltration hybrid system effectively removes virus that is difficult to remove in conventional coagulation-sedimentation-sand filtration process (2008)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Kobuke, M., Urasaki, T. and Ohno, K
  • Organizer: Proceedings of ICOM2008
  • Place of Presentation: Sheraton Waikiki, Honolulu, Honolulu, Hawaii USA
  • Year and Date: 20080712-18
• [Presentation] Difference in behaviors of two indicator bacteriophages for waterborne pathogenic viruses during drinking water treatment process, and their effective removals by using coagulation-ceramic microfiltration hybrid system (2008)
  • Author(s): Shirasaki, N., Kobuke, M., Urasaki, T., Matsushita, T., Matsui, Y., and Ohno, K
  • Organizer: Proceedings of IWA regional conference-membrane technologies in water and waste water treatment
  • Place of Presentation: Crocus International Exhibition Centre, Moscow, Russia
  • Year and Date: 20080602-04
• [Presentation] 浄水処理におけるバクテリオファージの処理性の違い (2008)
  • Author(s): 浦崎稔史, 白崎伸隆, 小泓誠, 松下拓, 松井佳彦, 大野浩一
  • Organizer: 第59回全国水道研究発表会
  • Place of Presentation: 仙台国際センター, 仙台
  • Year and Date: 20080523-25
• [Presentation] 凝集MF 膜処理によるウイルス除去に与えるファウリングの影響 (2007)
  • Author(s): 小泓誠, 白崎伸隆, 松下拓, 松井佳彦, 大野浩一
  • Organizer: 第15回衛生工学シンポジウム
  • Place of Presentation: 北海道大学,札幌
  • Year and Date: 20071108-09
• [Presentation] Effects of reversible and irreversible membrane fouling on virus removal by a coagulation-microfiltration system (2007)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y. and Ohno, K
  • Organizer: Proceedings of IWA International Conference on Particle Separation (PS 2007)
  • Place of Presentation: Universite Paul Sabatier, Toulouse, France
  • Year and Date: 20070709-12
• [Presentation] Virus removal in a hybrid coagulation-microfiltration system- Investigating mechanisms of virus removal by a combination of PCR and PFU methods (2007)
  • Author(s): Shirasaki, N., Matsushita, T., Matsui, Y., Ohno, K., Kobuke, M
  • Organizer: Proceedings of IWA 4th International Conference on Membranes for Water and Wastewater Treatment
  • Place of Presentation: HarrogateInternational Conference Centre, Harrogate, UK
  • Year and Date: 20070514-17