Environmental adaptation and body fluid homeostasis of fish by transporting epithelial cells

2008 Fiscal Year Final Research Report

• Project/Area Number: 19770057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: KATO Akira Tokyo Institute of Technology, 大学院・生命理工学研究科, 助教 (40311336)
• Project Period (FY): 2007 – 2008
• Keywords: 魚類生理 / 電気生理 / 体液ホメオスタシス / イオン輸送体 / フグゲノム / 重炭酸輸送体 / 硫酸輸送体 / 輸送上皮細胞

Research Abstract

魚類が淡水や海水の中で体液を人とほぼ同じ組成に保つ仕組みを, エラ, 腎臓, 腸によるイオン輸送の結果として理解することを目標に, トラフグ(ゲノム配列が公開)の近縁種で淡水・海水の両環境で生息できるメフグのイオン輸送体遺伝子を解析した。淡水魚が尿から水を排泄する仕組み, 海水魚が糞からカルシウムを排泄する仕組み, 及び海水魚が尿から硫酸イオンを排泄する仕組みに関わる遺伝子を明らかにした。

Research Products

• [Journal Article] Identification of intestinal bicarbonate transporters involved in formation of carbonate precipitates to stimulate water absorption in marine teleost fish (2008)
  • Author(s): Kurita Y, Nakada T, Kato A, Doi H, Mistry AC, Chang MH, Romero MF, Hirose S
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 294 Pages: R1402-12
• [Journal Article] Characterization of the column and autocellular junctions that define the vasculature of gill lamellae (2007)
  • Author(s): Kato A, Nakamura K, Kudo H, Tran YH, Yamamoto Y, Doi H, Hirose S
  • Journal Title: J Histochem Cytochem 55 Pages: 941-53
• [Journal Article] Fish calcitonin receptor has novel features (2007)
  • Author(s): Nag K, Kato A, Sultana N, Ogoshi M, Takei Y, Hirose S
  • Journal Title: Gen Comp Endocrinol 154 Pages: 48-58
• [Journal Article] Headless splice variant acting as dominant negative calcitonin receptor (2007)
  • Author(s): Nag K, Sultana N, Kato A, Hirose S
  • Journal Title: Biochem Biophys Res Commun 362 Pages: 1037-43
• [Journal Article] Ammonia secretion from fish gill depends on a set of Rh glycoproteins (2007)
  • Author(s): Nakada T, Westhoff CM, Kato A, Hirose S
  • Journal Title: FASEB J
• [Journal Article] Pillar cell and erythrocyte localization of fugu ET(A) receptor and its implication (2007)
  • Author(s): Sultana N, Nag K, Kato A, Hirose S
  • Journal Title: Biochem Biophys Res Commun
• [Presentation] 海水魚の硫酸排泄機構の解析 (2008)
  • Author(s): 加藤 明, 中田 勉, 栗田 志広, 土井 啓行, Min-Hwang Chang, Michael F. Romero, 広瀬茂久
  • Organizer: 第3回トランスポーター研究会
  • Place of Presentation: 京都
  • Year and Date: 2008-06-08
• [Presentation] Functional Characterization of Pufferfish Slc26a6A and Slc26a6B (2008)
  • Author(s): Kato A, Chang MH, Kurita Y, Nakada T, Hirose S, Romero MF
  • Organizer: Experimental Biology 2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-04-08
• [Presentation] フグSlc26陰イオン交換輸送体の活性と海水適応における役割 (2007)
  • Author(s): 加藤 明, Min-Hwang Chang, 栗田 志広, 中田 勉, 広瀬 茂久, Michael F. Romero
  • Organizer: トランスポーター研究会第1回関東部会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-10