2010 Fiscal Year Final Research Report
Crystallization and structural analysis of proton motor of ATP synthase
Project/Area Number |
19770103
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Functional biochemistry
|
Research Institution | Tokyo Institute of Technology |
Principal Investigator |
MITOME Noriyo Tokyo Institute of Technology, 物質工学科, 講師 (90431981)
|
Project Period (FY) |
2007 – 2010
|
Keywords | ATP合成酵素 / 構造解析 / 分子モーター / プロトンポンプ |
Research Abstract |
In FoF1-ATP synthase that couples H+ transport with ATP synthesis/hydrolysis, an Foc subunit decamer ring (c-ring) rotates relative to F_oa as protons pass through Fo. To understand the torque generation mechanism, structural and functional information is required. In this study, I generated c_<10>-aF_oF_1 in which c_<10> and F_oa were genetically fused. The biochemical analysis revealed that the conserved arginine residue in F_oa ensures proton-coupled c-ring rotation by preventing a futile proton shortcut. I also successfully purified c_<10>-a F_o and analyzed stability of complexes for crystallization of F_o.
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Research Products
(15 results)