2009 Fiscal Year Final Research Report
Fluctuation and Speed of Microtubule Movements Driven by Kinesin Motors Arranged on Catchin Thick Filaments
| Project/Area Number |
19770132
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Kyushu University |
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Principal Investigator |
IMAFUKU Yasuhiro Kyushu University, 大学院・理学研究院, 助教 (70304722)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 運動・輸送 / 分子モーター |
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| Research Abstract |
We arranged the motor domains of kinesin by using the assembly property of catchin, a myosin rod-like protein extracted from bivalve smooth muscles. We prepared kinesin-catchin chimera protein and attached them to the surface of catchin filaments. We analyzed speed and direction of the movements of microtubules on kinesin-chimera/catchin filament.
We also showed theoretically that thermally activated kinesin head detachment followed by rapid reattachment at another binding site on microtubule can cause the motor to 'hop' backwards without ATP hydrolysis.
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Research Products
(13 results)
