2009 Fiscal Year Final Research Report
Identification of activators for a functional DNA sequence that promotes replication initiation.
Project/Area Number |
19770150
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Molecular biology
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Research Institution | Kyushu University |
Principal Investigator |
FUJIMITSU Kazuyuki Kyushu University, 大学院・薬学研究院, 特任助教 (90448431)
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Project Period (FY) |
2007 – 2009
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Keywords | 核酸 / 遺伝子 / ゲノムDNA複製 / 蛋白質 / 活性制御 / 機能性DNA |
Research Abstract |
To study the mechanism for regulation of the initiation of DNA replication, I focused on a specific DNA segment that activates the initiator of E. coli chromosomal replication, DnaA. Our recent results suggested the presence of a soluble factor that can promote the activity of the DNA segment. Here, we reveal that two DNA binding proteins simultaneously function to stimulate the DNA segment in vitro and in vivo.
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[Journal Article] Modes of overinitiation, dnaA gene expression, and inhibition of cell division in a novel cold-sensitive hda mutant of Escherichia coli2008
Author(s)
Fujimitsu, K., Su'etsugu, M., Yamaguchi, Y., Mazda, K., Fu, N., Kawakami, H., Katayama, T.
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Journal Title
J Bacteriol 190巻
Pages: 5368-5381
Peer Reviewed
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[Journal Article] The interaction of DiaA and DnaA regulates the replication cycle in E. coli by directly promoting ATP DnaA-specific initiation complexes2007
Author(s)
Keyamura, K., Fujikawa, N., Ishida, T., Ozaki, S., Su'etsugu, M., Fujimitsu, K., Kagawa, W., Yokoyama, S., Kurumizaka, H., Katayama, T
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Journal Title
Genes & Development 21巻
Pages: 2083-2099
Peer Reviewed
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