• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2008 Fiscal Year Final Research Report

Studies of the role on dominant negative form Importin13 upon mouse meiotic germ cell development

Research Project

  • PDF
Project/Area Number 19770198
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionKumamoto University

Principal Investigator

YAMAGUCHI Yasuka  Kumamoto University, 発生医学研究センター, 研究員 (90448522)

Project Period (FY) 2007 – 2008
Keywords生殖細胞 / 減数分裂 / Ipo13 / マウス / 組織培養
Research Abstract

細胞分化等を誘導する分子の細胞内局在変化の引き金となるImportin ファミリーの一つ、Ipo13遺伝子の減数分裂期生殖細胞細胞分化における役割について研究を進めた。全長(long)のIpo13(L-Ipo13)は標的タンパク質カーゴ分子であり核内で活性を示すubiquitin-conjugating enzyme 9(UBC9)の核局在を誘導する。しかし、精巣特異的発現を示す、核移行領域欠損型のTestis specific(TS)-Ipo13はUBC9の核への局在化を誘導しなかった。さらに両者の共発現ではL-Ipo13のUBC9の輸送活性が阻害されることを観察した。また、UBC9が核局在を示さない条件下では、生殖細胞の減数分裂の進行が遅れる様子を観察した。以上の観察から、転写開始点の使い分けによって得られると推測されるTS-IPO13は、Ipo13遺伝子の全長産物であるL-IPO13の細胞質核間輸送活性を低下させ、UBC9などの標的カーゴ分子の核蓄積を阻害して、減数分裂期の生殖細胞の分化を制御していると結論づけた。

  • Research Products

    (8 results)

All 2008 2007 Other

All Presentation (7 results) Book (1 results)

  • [Presentation] Six1 and Six4 Homeoproteins are required for Sex Determination in Mice2008

    • Author(s)
      Satomi S. Tanaka, Yuka Fujimoto, Yasuka L. Yamaguchi, Hiroki Kobayashi and Ryuichi Nishinakamura
    • Organizer
      International symposium for gonad and brain sex differentiation
    • Place of Presentation
      LAL resort Sea Hawk hotel Fukuoka, Fukuoka
    • Year and Date
      20080914-16
  • [Presentation] Dullard is essential for primordial germ cell formation and posterior extraembryonic mesoderm differentiation in the gastrulating mouse embryo2008

    • Author(s)
      Satomi S. Tanaka, Akihiro Nakane, Yasuka Y. Yamaguchi, Sayoko Fujimura, Makoto Asashima, Ryuichi Nishinakamura
    • Organizer
      Annual meeting for the Japanese Society of Developmental Biologists (jointly sponsored by International Society of Developmental Biologists)
    • Place of Presentation
      Tokushima Arts Foundation for Culture, Tokushima
    • Year and Date
      20080528-30
  • [Presentation] Stage-specific Importin13 activity regulates meiosis of germ cells in the mouse2008

    • Author(s)
      Yasuka L. Yamaguchi et al.
    • Organizer
      Annual meeting for the Japanese Society of Developmental Biologists(jointly sponsored by International Society of Developmental Biologists)
    • Place of Presentation
      Tokushima Arts Foundation for Culture, Tokushima
    • Year and Date
      20080528-30
  • [Presentation] Sall4 is essential for development of mouse primordial germ cells2008

    • Author(s)
      Yasuka L. Yamaguchi et al.
    • Organizer
      The 23rd Naito Conference,Molecular Basis for Maintenance and Differentiation of Stem Cells [III]
    • Place of Presentation
      Shonan Village Center, Kanagawa, Japan
    • Year and Date
      20080111-14
  • [Presentation] マウス生殖細胞発生におけるSall4遺伝子の分子機構の解析2007

    • Author(s)
      Yasuka L. Yamaguchi et al.
    • Organizer
      80th Annual Meeting for the Japanese Biochemical Society(Biochemistry and Molecular Biology 2007, BMB2007)
    • Place of Presentation
      Pacifico Yokohama, Yokohama, Japan
    • Year and Date
      20071211-15
  • [Presentation] マウス生殖細胞形成・分化におけるSall4遺伝子の分子機構の解析2007

    • Author(s)
      Yasuka L. Yamaguchi et al.
    • Organizer
      40th Annual Meeting for the Japanese Society of Developmental Biologists 2007(JSDB2007)
    • Place of Presentation
      Fukuoka Dome, Fukuoka, Japan
    • Year and Date
      20070528-30
  • [Presentation] Sall4 is required for mouse primordial germ cell development

    • Author(s)
      Yasuka L. Yamaguchi, Satomi S. Tanaka, Ryuichi Nishinakamura
    • Organizer
      COE Mini Symposium, February 19th 2009
    • Place of Presentation
      Kumamoto Univ., Kumamoto. oral presenter
  • [Book] Analyzing gene function in whole mouse embryo and fetal organ in vitro

    • Author(s)
      Satomi S. Tanaka, Yasuka L. Yamaguchi, Vanessa J. Jones and Patrick P. L. Tam.
    • Publisher
      In Methods in Molecular Biology (M. Lewandoski ed.), Humana Press

URL: 

Published: 2010-06-10   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi