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2008 Fiscal Year Final Research Report

Molecular basis of one-carbon metabolism of microorganisms and the regulatory mechanism of the gene expression

Research Project

  • PDF
Project/Area Number 19780058
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Applied microbiology
Research InstitutionKyoto University

Principal Investigator

YURIMOTO Hiroya  Kyoto University, 大学院・農学研究科, 准教授 (00283648)

Project Period (FY) 2007 – 2008
Keywordsメタノール / ホルムアルデヒド / Candida boidinii / プロモーター / リブロースモノリン酸経路 / Mycobacterium gastri / HPS / PHI
Research Abstract

酵母メタノール誘導性遺伝子発現に関わる転写因子を新たに取得し、Trm1 がメタノール特異的誘導、Trm2 がグルコース脱抑制に関わる転写因子として機能していることを明らかにした。枯草菌のホルムアルデヒド応答性遺伝子発現制御機構を利用し、ホルムアルデヒドを検出できるセンサー細胞を開発した。メタノール資化性細菌のホルムアルデヒド固定経路の酵素(HPS, PHI)を人工的に融合した二機能性酵素を創製し、ホルムアルデヒド資化性や耐性を異種生物に付与することに成功した。

  • Research Products

    (9 results)

All 2009 2008 2007

All Journal Article (9 results) (of which Peer Reviewed: 8 results)

  • [Journal Article] Activation of the oxidative stress regulator PpYap1 through conserved cysteine residues during methanol metabolism in the yeast Pichia pastoris2009

    • Author(s)
      Yano, T., H. Yurimoto, Y. Sakai
    • Journal Title

      Biosci. Biotechnol. Biochem. (in press)

    • Peer Reviewed
  • [Journal Article] Methanolinducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii2009

    • Author(s)
      Yurimoto H., Y. Sakai
    • Journal Title

      Biotechnol. Appl. Biochem. 53

      Pages: 85-92

    • Peer Reviewed
  • [Journal Article] Molecular basis of methanol-inducible gene expression and its application in the methylotrophic yeast Candida boidinii2009

    • Author(s)
      Yurimoto, H.
    • Journal Title

      Biosci. Biotechnol. Biochem. 73

      Pages: 793-800

    • Peer Reviewed
  • [Journal Article] The Yap-1 regulated glutathione redox system curtails the accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia pastoris2009

    • Author(s)
      Yano, T., E. Takigami, H. Yurimoto, Y. Sakai.
    • Journal Title

      Eukaryot. Cell 8

      Pages: 540-549

    • Peer Reviewed
  • [Journal Article] Overexpression and purification of rat peroxisomal membrane protein 22, PMP22, in Pichia pastoris2009

    • Author(s)
      Egawa, K., H. Shibata, S. Yamashita, H. Yurimoto, Y. Sakai, H. Kato
    • Journal Title

      Protein Expr. Purif. 64

      Pages: 47-54

    • Peer Reviewed
  • [Journal Article] アーキアにおけるホルムアルデヒド固定酵素群の生理機能2008

    • Author(s)
      由里本博也, 折田和泉, 阪井康能
    • Journal Title

      バイオサイエンスとインダストリー 66

      Pages: 447-449

  • [Journal Article] Trm1p, a Zn(II)_2Cys_6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii2008

    • Author(s)
      Sasano, Y., H. Yurimoto, M. Yanaka, Y. Sakai.
    • Journal Title

      Eukaryot. Cell. 7

      Pages: 527-536

    • Peer Reviewed
  • [Journal Article] Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase2007

    • Author(s)
      Orita, I., N. Sakamoto, N. Kato, H. Yurimoto, Y. Sakai
    • Journal Title

      Appl. Microbiol. Biotechnol. 76

      Pages: 439-445

    • Peer Reviewed
  • [Journal Article] Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii2007

    • Author(s)
      Sasano, Y., H. Yurimoto, Y. Sakai
    • Journal Title

      J. Biosci. Bioeng. 104

      Pages: 86-89

    • Peer Reviewed

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Published: 2010-06-10   Modified: 2016-04-21  

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