2009 Fiscal Year Final Research Report
Effect of intramammary infusion of recombinant bovine cytokines on CMT score and milk mononuclear cell populations in Holstein cows with subclinical mastitis
| Project/Area Number |
19780236
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Clinical veterinary science
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| Research Institution | National Agricultural Research Organization |
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Principal Investigator |
KIKU Yoshio National Agricultural Research Organization, 動物衛生研究所, 主任研究員 (70370179)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 治療 / 乳房炎 / サイトカイン |
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| Research Abstract |
The effect of intramammary infusion of recombinant bovine (rb) cytokine on mononuclear cell populations in udders, and CMT score was investigated. Twenty-one cows with naturally occurring subclinical mastitis were infused rbGM-CSF, rbIL-8 and the combination of the two into the udders. Milk samples were collected from the udders on days 0, 1/4, 1, 2, 3, 7 and 14 after infusions of all cytokines. The CMT score sharply rose between days 1/4 and 2, and was followed by a smooth decline to low pre-infusion levels between days 7 and 14. Interestingly, the percentage of CD14+ cells increased on days 1 and 2 after infusion of rbGM-CSF only. Subsequent to this increase in CD14+ cells, CD4+ and CD8+ cells also increased on days 2 and 3, suggesting that the infusion of rbGM-CSF enhanced cellular immunity in the mammary gland. In contrast, the percentages of CD4+ and CD8+ cells decreased on days 1/4 and 1 after infusion of rbIL-8 and the combination respectively. However, the mechanism of this decrease following the infusion of the two is not understood and this calls for future work in this area. These results demonstrate that the intramammary infusion of rbGM-CSF induces the alteration of mononuclear cell populations in milk, and that it may have potential as a therapeutic agent for subclinical mastitis of dairy cows.
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