The analysis of cell-cell barrier function in intestine

2009 Fiscal Year Final Research Report

• Project/Area Number: 19790150
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Osaka University
• Principal Investigator: TAMURA Atsushi Osaka University, 生命機能研究科, 特任助教 (00362525)
• Project Period (FY): 2007 – 2009
• Keywords: タイトジャンクション / クローディン / ノックアウトマウス / 腸管 / ホメオスタシス

Research Abstract

Epithelial cell sheets have an important role in compartmentalizing internal tissues and maintaining their local homeostasis via intercellular barriers consisting of tight junctions. Using claudin-15 knockout mice, we proposed that claudin-15-based formation of tight junctions to organize was important for the ion like Na+ microenvironment in the small intestine. As the absorption of many kinds of nutrients like glucose is coupled directly to Na+ absorption, this result is essential for our life.

Research Products

• [Journal Article] Megaintestine in claudin-15- deficient mice. (2008)
  • Author(s): Tamura, A., Kitano, Y., Hata, M., Katsuno, T., Moriwaki, K., Sasaki, H., Hayashi, H, Suzuki, Y., Noda, T., Furuse, M., Tsukita, Sh., Tsukita. Sa.
  • Journal Title: Gastroenterology 134 Pages: 523-534
  • Peer Reviewed
• [Journal Article] Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells. (2008)
  • Author(s): Katsuno, T., Umeda, K., Matsui, T., Hata, M., Tamura, A., Itoh, M., Takeuchi, K., Fujimori, T., Nabeshima, Y., Noda, T., Tsukita, Sh., Tsukita. Sa.
  • Journal Title: Mol. Biol. Cell 19 Pages: 2465-2475
  • Peer Reviewed
• [Presentation] 細胞間隙イオン輸送担体としての細胞間接着分子クローディン (2009)
  • Author(s): 月田早智子、山崎裕自、田村淳
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸、日本
  • Year and Date: 2009-10-21
• [Presentation] 細胞間カチオンチャネルとしてのクローディンノックアウトマウスの解析から (2009)
  • Author(s): 田村淳
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸、日本
  • Year and Date: 2009-10-21
• [Presentation] Paracellular Na+-permeative Claudin-15-2 based homeostasis, proliferation, and inflammation in mouse intestine. (2009)
  • Author(s): Atsushi Tamura, Mitsunobu Imazato, Yuji Yamazaki, Mikio Furuse, Tetsuo Noda, Shoichiro Tsukita, Sachiko Tsukita
  • Organizer: ASCB/JSCB/RIKEN CDB 2009 Summer Meeting Building the Body Plan: How Cell Adhesionm Signaling and Cytoskeletal Regulation Shape Morphogenesis
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-09-21
• [Presentation] Role of claudin-15 and related claudins: Knockout-mice analyses (2009)
  • Author(s): Sachiko Tsukita, Atsushi Tamura, Shoichiro Tsukita
  • Organizer: The 36th International Congress of Physiological Sciences (IUPS2009)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-30
• [Presentation] 上皮細胞特有の細胞間接着性の特性と細胞増殖 (2008)
  • Author(s): 月田早智子、野島久、田村淳
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋、日本
  • Year and Date: 2008-10-28