2009 Fiscal Year Final Research Report
Molecular Machineries of Signaling in Kidney Macula Densa.
| Project/Area Number |
19790169
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Kitasato University |
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Principal Investigator |
YASUOKA Yukiko Kitasato University, 医学部, 助教 (50348504)
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| Project Period (FY) |
2007 – 2009
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| Keywords | マクラデンサ / NKCC2 / nNOS |
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| Research Abstract |
Changes in the luminal NaCl concentration ([NaCl]) at the kidney macula densa (MD) modulate the tubuloglomerular feedback (TGF) responses via affecting the release of nitric oxide (NO). In a newly established mouse macula densa cell line (NE-MD), we demonstrated that furosemide, an inhibitor of Na^+-K^+-2Cl^- cotransporter, and low [Cl^-] similarly induced increases in both the nNOS protein expression and the L-Arg-induced NO generation. We also found that the nNOS-like protein (60kD) determined in the NE-MD cells was smaller in size than the usual nNOS proteins (160kD) obtained from brain and skeletal muscle. Molecular structure of the NE-MD nNOS protein was carefully analyzed by the two-dimensional electrophoresis and the mass spectrometry (MALDI-TOF-MS). Based on the observation of the MS analysis of the peptide piece, we conclude that a main part of the nNOS reduction domain has been deleted in the furosemide-induced nNOS protein of NE-MD.
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[Journal Article] Vasopressin regulates the renin- angiotensin- aldosterone system via V1a receptors in macula densa cells.2008
Author(s)
Aoyagi T, Izumi Y, Hiroyama M, Matsuzaki T, Yasuoka Y, Sanbe A, Miyazaki H, Fujiwara Y, Nakayama Y, Kohda Y, Yamauchi J, Inoue T, Kawahara K, Saito H, Tomita K, Nonoguchi H, Tanoue A
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Journal Title
Am J Physiol Renal Physiol 295
Pages: F100-7
Peer Reviewed
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