2009 Fiscal Year Final Research Report
Functional analysis of phospholipase C-zeta-deficient mouse
| Project/Area Number |
19790170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Yamaguchi University (2009)
Tokyo Women's Medical University (2007-2008) |
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Principal Investigator |
ITO Masahiko Tokyo Women's Medical University, 総合科学実験センター, 助教 (50385423)
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAZAKI Shunichi 東京女子医科大学, 学長 (80010081)
MIYADO Kenji 国立成育医療研究センター研究所, 生殖医療研究部, 室長 (60324844)
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| Project Period (FY) |
2007 – 2009
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| Keywords | ホスホリパーゼCゼータ / 卵活性化精子因子 / カルシウムオシレーション / 遺伝子欠損マウス / 精子形成 |
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| Research Abstract |
In mammalian fertilization, Ca^<2+> oscillations are indispensable for egg- activation. Sperm specific isotype of phospholopase C (PLCZ1) is a strong candidate for egg-activating sperm factor. In this study, regulation of Ca^<2+> oscillations by PLCZ1 was examined about nuclear localization ability and Ca^<2+> oscillation inducing activity. Moreover, PLCZ1 deficient mice were analyzed and it was shown that PLCZ1 has a pivotal role for spermatogenesis as well as an egg activation.
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[Journal Article] Difference in Ca2+ oscillation - inducing activity and nuclear translocation ability of PLCZ1, an egg - activating sperm factor candidate, among mouse, rat, human and medaka fish.2008
Author(s)
Masahiko Ito, Tomohide Shikano, Shoji Oda, Takashi Horiguchi, Satomi Tanimoto, Takeo Awaji, Hiroshi Mitani, Shunichi Miyazaki.
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Journal Title
Biol Reprod 78
Pages: 1081-1090
Peer Reviewed
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