Study on the physiological role of signal adaptor DAP10 in osteoclast development

2008 Fiscal Year Final Research Report

• Project/Area Number: 19790203
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Wakayama Medical University
• Principal Investigator: INUI Masanori Wakayama Medical University, 医学部, 助教 (80443985)
• Project Period (FY): 2007 – 2008
• Keywords: 骨免疫学 / 骨代謝学 / 免疫学

Research Abstract

DAP10遺伝子欠損マウスの解析において,DAP10遺伝子欠損マウスが加齢に伴う破骨細胞の分化障害により骨硬化を呈することを明らかにした.さらに,破骨細胞におけるDAP10会合分子の探索を行ったところ,Myeloid DAP12-associating lectin (MDL)-1を同定し, MDL-1/DAP10/DAP12の三量体を形成することを示した.このようにDAP10はMDL-1を介してDAP12と協調的にはたらき,破骨細胞分化を制御していることを明らかにした.

Research Products

• [Journal Article] Signal adaptor DAP10 associates with MDL-1 and triggers osteoclastogenesis in cooperation with DAP12 (2009)
  • Author(s): Inui M, Kikuchi Y, Aoki N, Endo S, Maeda T, Sugahara-Tobinai A, Fujimura S, Nakamura A, Kumanogoh A, Colonna M, Takai T
  • Journal Title: Proc. Natl. Acad. Sci. U. S. A 106 Pages: 4816-4821
  • Peer Reviewed
• [Journal Article] Inhibitory immunoglobulin-like receptors LILRB and PIR-B negatively regulate osteoclast development (2008)
  • Author(s): Mori Y, Tsuji S, Inui M, Sakamoto Y, Endo S, Ito Y, Fujimura S, Koga T, Nakamura A, Takayanagi H, Itoi E, Takai T
  • Journal Title: J. Immunol 181 Pages: 4742-4751
  • Peer Reviewed
• [Journal Article] A novel regulatory role of gp49B on dendritic cells in T cell priming. Eur (2008)
  • Author(s): Kasai S, Inui M, Nakamura K, Kakizaki Y, Endo S, Nakamura A, Ito S, Takai T
  • Journal Title: J. Immunol 38 Pages: 2426-2437
  • Peer Reviewed
• [Journal Article] The adaptor protein Card9 is essential for the activation of myeloid cells through ITAM-associated and Toll-like receptors (2007)
  • Author(s): Hara H, Ishihara C, Takeuchi A, Imanishi T, Xue L, Morris S, Inui M, Takai T, Shibuya A, Saijo S, Iwakura Y, Ohno N, Koseki H, Yoshida H, Penninger J, Saito T
  • Journal Title: Nat. Immunol 8 Pages: 619-629
  • Peer Reviewed
• [Presentation] Protective role of CX3CR1-mediated signal phathway in clostridium difficile toxin A-induced enteritis (2008)
  • Author(s): Masanori Inui, Yuko Ishida, Akihiko Kimura, Naofumi Mukaida, Toshikazu Kondo
  • Organizer: Cytokines 2008
  • Place of Presentation: Montreal, Canada
  • Year and Date: 20080000
• [Presentation] Protective role of CX3CR1-mediated signal phathway in clostridium difficile toxin A-induced enteritis (2008)
  • Author(s): Masanori Inui, Yuko Ishida, Akihiko Kimura, Naofumi Mukaida, Toshikazu Kondo
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 20080000
• [Presentation] Signal adaptor DAP10 associates with MDL-1 and triggers osteoclastogenesis in cooperation with DAP12 (2008)
  • Author(s): Fujimura Shion, Inui Masanori, Kikuchi Yuki, Aoki Naoko, Endo Shota, Maeda Tsutomu, Sugahara-Tobinai Akiko, Nakamura Akira, Kumanogoh Atsushi, Colonna Marco, Takai Toshiyuki
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 20080000
• [Presentation] MRL/lprマウスのエリテマトーデス様皮膚症に対する紫外線UVA-1治療の予防的効果の検討 (2008)
  • Author(s): 三木田直哉,李洪錦,乾匡範,池田高治,吉益隆,金澤伸雄,古川福実
  • Organizer: 日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 20080000
• [Presentation] ITAM型免疫レセプターによる骨ホメオスターシスの制御 (2008)
  • Author(s): 高井俊行,乾匡範,藤村紫音,遠藤章太,中村晃
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Presentation] MDL-1-DAP10 complex triggers osteoclastogenesis in cooperation with DAP12 (2008)
  • Author(s): Fujimura Shion, Inui Masanori, Kikuchi Yuki, Aoki Naoko, Endo Shota, Maeda Tsutomu, Sugahara-Tobinai Akiko, Nakamura Akira, Kumanogoh Atsushi, Colonna Marco, Takai Toshiyuki
  • Organizer: BMB2008
  • Place of Presentation: 神戸
  • Year and Date: 20080000
• [Book] 免疫グロブリン様受容体と骨代謝.腎と骨代謝21 (2008)
  • Author(s): 高井俊行, 乾匡範, 森優
  • Total Pages: 43-50
• [Book] 細胞・培地活用ハンドブック骨免疫学研究 (2008)
  • Author(s): 乾匡範
  • Total Pages: 212-226
  • Publisher: 羊土社