Biological roles of REST in non-neuronal cells and its dysregulation in tumorgenesis

2008 Fiscal Year Final Research Report

• Project/Area Number: 19790267
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Human pathology
• Research Institution: Nagasaki University (2008); Iwate Medical University (2007)
• Principal Investigator: SHIBAZAKI Masahiko Nagasaki University, 大学院・医歯薬総合研究科, 助教 (20445109)
• Project Period (FY): 2007 – 2008
• Keywords: βIII-tubulin / REST / タキソール薬剤耐性機構 / 細胞周期 / 悪性腫瘍

Research Abstract

細胞骨格因子β-tubulin,特にβIII-tubulin(以下βIII)は神経細胞特異的である.しかし多く腫瘍ではβIIIの異所的発現が見られ,タキソール系抗がん剤に対し抵抗性を獲得する.本研究ではβIII遺伝子の発現制御因子RESTに注目し,異所的βIII発現機構,さらに薬剤耐性機構を検討した.その結果,RESTが細胞周期依存的に変動しβIIIを産生すること,また悪性黒色腫細胞でβIIIの発現が低いものは,タキソールに高感受性であることを明らかにし,一部の悪性黒色腫に対するタキソールの有効性を示唆した.

Research Products

• [Journal Article] Loss of Class III β-Tubulin Induced by Histone Deacetylation Is Associated with Chemosensitivity to Paclitaxel in Malignant Melanoma Cells (2009)
  • Author(s): Akasaka K, Maesawa C, Shibazaki M, Maeda F, Takahashi K, Akasaka T, Masuda
  • Journal Title: Journal of Investigative Dermatology (in press)
  • Peer Reviewed
• [Journal Article] Epigenetic modification is involved in aberrant expression of class III β-tubulin, TUBB3, in ovarian cancer cells (2008)
  • Author(s): Izutsu N, Maesawa C, Shibazaki M, Oikawa H, Shoji T, Sugiyama T, Masuda T
  • Journal Title: International Journal of Oncology 32 Pages: 1227-1235
  • Peer Reviewed
• [Journal Article] Downregulation of miR-138 is associated with overexpression of human telomerase reverse transcriptase protein in human anaplastic thyroid carcinoma cell lines (2008)
  • Author(s): Mitomo S, Maesawa C, Ogasawara S, Iwaya T, Shibazaki M, Yashima-Abo A, Kotani K, Oikawa H, Sakurai E, Izutsu N, Kato K, Komatsu H, Ikeda K, Wakabayashi G, Masuda T
  • Journal Title: Cancer Science 99 Pages: 280-286
  • Peer Reviewed