Development of a screening method for drugs in formalin-fixed tissue

2009 Fiscal Year Final Research Report

• Project/Area Number: 19790451
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Legal medicine
• Research Institution: National Research Institute of Police Science
• Principal Investigator: TSUJIKAWA Kenji National Research Institute of Police Science, 法科学第三部化学第一研究室, 研究員 (50356193)
• Project Period (FY): 2007 – 2009
• Keywords: 法医鑑定学 / 薬毒物スクリーニング

Research Abstract

The aim of this study is to develop a screening method for 38 central nervous system drugs such as hypnotics, antidepressants, antipsychotics. The drug standards were spiked to methanol homogenate of porcine liver, then clean-up was performed as following order : i) fat precipitation by cooling, ii) hexane-acetonitrile partition, iii) liquid-liquid extraction under basic condition. The sample prepared was determined by liquid chromatography tandem mass spectrometry. The recovery and repeatability (CV%) of the targeted drugs except for 7-aminobenzodiazepines (3 drugs) were more than 40% and within 15%, respectively. The method would be useful for drug screening in solid tissues.