2008 Fiscal Year Final Research Report
Identification of DC-STAMP ligand and clarification of the mechanisms of cell-cell fusion
| Project/Area Number |
19791044
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Keio University |
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Principal Investigator |
MIYAMOTO Takeshi Keio University, 医学部, 准教授 (70383768)
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| Project Period (FY) |
2007 – 2008
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| Keywords | 骨 / 軟骨代謝学 |
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| Research Abstract |
細胞融合因子DC-STAMPのリガンドの同定、およびDC-STAMPを介した細胞融合機構を解明するため、本研究は施行された。DC-STAMPを全身性に過剰に発現するトランスジェニックマウスを作製し、その表現型を解析することで、DC-STAMPが組織特異的に細胞融合を制御することなどを明らかにすることができた。また、DC-STAMPのリガンドの候補としてMCP-1を同定し、MCP-1欠損マウスを用いて多核の破骨細胞分化についての解析を行った。さらに、DC-STAMPの発現制御から細胞融合制御を解明するため、破骨細胞ならびにマクロファージ巨細胞におけるDC-STAMPの転写因子による発現制御機構の解明を行った。
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[Journal Article] The role of DC-STAMP in maintenance of immune tolerance through regulation of dendritic cell function2008
Author(s)
Sawatani Y, Miyamoto T, Nagai S, Maruya M, Imai J, Miyamoto K, Fujita N, Ninomiya K, Suzuki T, Iwasaki R, Toyama Y, Shinohara M, Koyasu S and Suda T
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Journal Title
Int Immunol 20(10)
Pages: 1259-1268
Peer Reviewed
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[Journal Article] Vascular endothelial growth factor-A is a survival factor for nucleus pulposus cells in the intervertebral disc2008
Author(s)
Fujita N, Imai J, Suzuki T, Yamada M, Ninomiya K, Miyamoto K, Iwasaki R, Morioka H, Matsumoto M, Chiba K, Watanabe S, Suda T, Toyama Y, Miyamoto T
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Journal Title
Biochem Biophys Res Commun 372(2)
Pages: 367-372
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[Journal Article] A Functional Polymorphism in THBS2 that Affects Alternative Splicing and MMP Binding Is Associated with Lumbar-Disc Herniation2008
Author(s)
Hirose Y, Chiba K, Karasugi T, Nakajima M, Kawaguchi Y, Mikami Y, Furuichi T, Mio F, Miyake A, Miyamoto T, Ozaki K, Takahashi A, Mizuta H, Kubo T, Kimura T, Tanaka T, Toyama Y, Ikegawa S
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Journal Title
Am J Hum Genet 82(5)
Pages: 1122-1129
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[Journal Article] Cell surface colony-stimulating factor 1 can be cleaved by TNF-alpha converting enzyme or endocytosed in a clathrin-dependent manner2007
Author(s)
Horiuchi K, Miyamoto T, Takaishi H, Hakozaki A, Kosaki N, Miyauchi Y, Furukawa M, Takito J, Kaneko H, Matsuzaki K, Morioka H, Blobel CP, Toyama Y
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Journal Title
J Immunol 179(10)
Pages: 6715-6724
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[Journal Article] Reactive oxygen species induce chondrocyte hypertrophy in endochondral ossification2007
Author(s)
Morita K, Miyamoto T, Fujita N, Kubota Y, Ito K, Takubo K, Miyamoto K, Ninomiya K, Suzuki T, Iwasaki R, Yagi M, Takaishi H, Toyama Y and Suda T
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Journal Title
J Exp Med 204(7)
Pages: 1613-1323
Peer Reviewed
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[Journal Article] Foxo3a is essential for maintenance of hematopoietic stem cell pool2007
Author(s)
Miyamoto K, Araki KY, Naka K,Arai F, Takubo K, Yamazaki S, Matsuoka S, Miyamoto T, Ito K, Ohmura M, Chen C, Hosokawa K, Nakauchi H, Nakayama K, Nakayama K, Harada M, Motoyama N, Suda T and Hirao A
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Journal Title
Cell Stem Cell 1(1)
Pages: 101-112
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[Presentation] IFN-beta Is a Key Molecule in Inhibition of Human Osteoclast Differentiation by 1alpha, 25-dihydroxyvitamin D32008
Author(s)
S. Sakai, H. Takaishi, K. Matsuzaki, H. Kaneko, M. Furukawa, A. Shiraishi, T. Suda, T. Miyamoto, Y. Toyama
Organizer
30th Annual Meeting of the American Society for Bone and Mineral Research
Place of Presentation
Palais des congres de Montreal, Montreal, Quebec, Canada
Year and Date
20080912-16
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[Presentation] Loss of Single Bim Allele Recovers the Defective Osteoclast Function in bcl2-/- Mice but Dose not Restore the Anabolic Action of PTH2008
Author(s)
Y. Nagase, M. Iwasawa, T. Akiyama, Y. Kadono, N. Ogata, Y. Oshima, M. Nakamura, T. Yasui, T. Miyamoto, K. Nakamura, S. Tanaka
Organizer
30th Annual Meeting of the American Society for Bone and Mineral Research
Place of Presentation
Palais des congres de Montreal, Montreal, Quebec, Canada
Year and Date
20080912-16
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[Presentation] Regulation of Skeletal Homeostasis by Anti-apoptotic Molecule Bcl-22007
Author(s)
Y. Nagase, M. Iwasawa, T. Akiyama, Y. Kadono, T. Miyamoto, Y. Oshima, M. Nakamura, T. Yasui, K. Nakamura, S. Tanaka
Organizer
29th Annual Meeting of the American Society for Bone and Mineral Research
Place of Presentation
Hawaii Convention Center, Honolulu, Hawaii
Year and Date
20070916-19
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