2009 Fiscal Year Final Research Report
Influence on prostate cancer of bone marrow stem cell (pre-adipocytes) :Comprehensive analysis of the signaling pathways
| Project/Area Number |
19791115
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | Saga University |
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Principal Investigator |
SATOH Yuji Saga University, 医学部, 講師 (40363436)
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| Project Period (FY) |
2007 – 2009
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| Keywords | 前立腺癌 / 脂肪細胞 / 3次元培養 / シグナル伝達 / 免疫染色 / Western Blot法 / Real-Time RT PCR / cDNA microarray |
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| Research Abstract |
We examined signaling pathways with human prostate cancer cell line and pre-adipocytes .Cancer cells were co-cultured with the isolated mature adipocytes in 3-D collagen gel matrix culture. In LNCaP, humoral factors and cell to cell contaction are necessary for proliferation. In PC-3, cell to cell contaction is necessary for differentiation. In DU145, humoral factors and cell to cell contaction are necessary for proliferation and differentiation. As a result of cDNA microarray, Prostate-specific expression PSGR2 and integrin significantly increased.
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