2009 Fiscal Year Final Research Report

Differential induction and application to regenerative medicine of ocular tissue-specific stem cells

Research Project

Project/Area Number	19791266
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Ophthalmology
Research Institution	Osaka University
Principal Investigator	INOUE Tomoyuki Osaka University, 医学系研究科, 助教 (50432539)
Project Period (FY)	2007 – 2009
Keywords	再生医療 / 眼組織 / 網膜 / 角膜
Research Abstract	For clinical application of regenerative medicine against severe ocular diseases, we investigated the ocular tissue-specific stem cells as a cell source for the target cells. We shown that modulation of retinal transcription factor gene expression in human RSCs greatly enriches for photoreceptor progeny, and that strong enrichment was obtained with the combined transduction of OTX2 and CRX together with the modulation of CHX10. These results were published on Stem Cells 2010 ; 28 : 489-500. In corneal regeneration therapy, we investigated the feeder cells for the cultivated cell sheet transplantation.