Identification of osteoclast niche using RANKL-deficient and M-CSF-deficient mice

2008 Fiscal Year Final Research Report

• Project/Area Number: 19791376
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: NAKAMICHI Yuko Matsumoto Dental University, 総合歯科・医学研究所, 助教 (20350829)
• Project Period (FY): 2007 – 2008
• Keywords: M-CSF / RANKL / 破骨細胞ニッチ / IL-34 / 活性型ビタミンD3

Research Abstract

細胞周期が停止した破骨細胞前駆細胞(pOCP ; post-mitotic osteoclast precursors)は、破骨細胞ニッチを形成する。pOCPの存在部位(すなわち破骨細胞ニッチ)を同定するため、pOCPをGFP標識できるトランスジェニック(Tg)マウスを開発するため、DNAコンストラクトを作製した。また本研究によりM-CSF受容体の新しいリガンドIL-34が、破骨細胞ニッチからの破骨細胞供給に関与することを示唆するデータを得た。

Research Products

• [Journal Article] Dental pulp and periodontal ligament cells support osteoclast differentiation (2009)
  • Author(s): Uchiyama M, Nakamichi Y, Nakamura M, Kinugawa S, Yamada H, Udagawa N, Miyazawa H.
  • Journal Title: J Dent Res (in press)
  • Peer Reviewed
• [Journal Article] Evaluation of Pharmaceuticals With a Novel Fifty-Hour Animal Model of Bone Loss (2009)
  • Author(s): Tomimori Y, Mori K, Koide M, Nakamichi Y, Ninomiya T, Udagawa N, Yasuda H.
  • Journal Title: J Bone Miner Res (in press)
  • Peer Reviewed
• [Journal Article] Identification of cell cycle-arrested quiescent osteoclast precursors in vivo (2009)
  • Author(s): Mizoguchi T, Muto A, Udagawa N, Arai A, Yamashita T, Hosoya A, Ninomiya T, Nakamura H, Yamamoto Y, Kinugawa S, Nakamura M, Nakamichi Y, Kobayashi Y, Nagasawa S, Oda K, Tanaka H, Tagaya M, Penninger JM, Ito M, Takahashi N.
  • Journal Title: J Cell Biol 184 Pages: 541-54
  • Peer Reviewed
• [Journal Article] Docetaxel inhibits bone resorption through suppression of osteoclast formation and function in different manners (2009)
  • Author(s): Takahashi M, Mizoguchi T, Uehara S, Nakamichi Y, Yang S, Naramoto H, Yamashita T, Kobayashi Y, Furusawa K, Udagawa N, Uematsu T, Takahashi N.
  • Journal Title: Bone Miner Metab 27 Pages: 24-35
  • Peer Reviewed
• [Journal Article] Altered fracture callus formation in chondromodulin-I deficient mice (2008)
  • Author(s): Yukata K, Matsui Y, Shukunami C, Takimoto A, Goto T, Nishizaki Y, Nakamichi Y, Kubo T, Sano T, Kato S, Hiraki Y, Yasui N.
  • Journal Title: Bone 43 Pages: 1047-56
  • Peer Reviewed
• [Presentation] An M-CSF-independent mechanism for osteoclastogenesis (2008)
  • Author(s): 中道裕子
  • Organizer: 第5回Bone Biology Forum
  • Place of Presentation: 静岡
  • Year and Date: 20080800
• [Presentation] M-CSF independent mechanisms for osteoclastogenesis (2007)
  • Author(s): Nakamichi Y, Udagawa N, Yasuda H, Nakamura M, Kobayashi Y, Takahashi N Nakamichi Y, Udagawa N, Yasuda H, Nakamura M, Kobayashi Y, Takahashi N
  • Organizer: (J Bone Miner Res 22, Suppl 1; S380, 2007) 29th American Society fo Bone and Mineral Research (ASBMR) Annual Meeting
  • Place of Presentation: モントリオール
  • Year and Date: 20070900
• [Presentation] M-CSF非存在下における破骨細胞形成メカニズムの解析 (2007)
  • Author(s): 中道裕子,宇田川信之,林眞一,保田尚孝,中村美どり,小林泰浩,高橋直之
  • Organizer: (第25回日本骨代謝学会プログラム抄録集: p.228)日本骨代謝学会(第25回)
  • Place of Presentation: 大阪
  • Year and Date: 20070700
• [Presentation] In vivoにおいて細胞周期の停止した破骨細胞前駆細胞(pOCP)は5-FU悲感受性である (2007)
  • Author(s): 溝口利英,武藤昭紀,細矢明宏,中道裕子,山下照仁,小林泰浩,宇田川信之,伊藤充雄,高橋直之
  • Organizer: (第25回日本骨代謝学会プログラム抄録集: p.275)日本骨代謝学会(第25回)
  • Place of Presentation: 大阪
  • Year and Date: 20070700
• [Remarks]
  • URL: http://www.mdu.ac.jp/laboratory/research_contents/index.html