大脳辺縁系および脳幹における急性ストレス反応

2008 Fiscal Year Annual Research Report

• Project/Area Number: 19890189
• Research Institution: Osaka City University
• Principal Investigator: 権 力 Osaka City University, 大学院・医学研究科, 助教 (60453130)
• Keywords: ストレス反応 / 大脳辺縁系 / 脳幹 / 神経病理 / 神経化学 / エリスローポエチン / C反応性蛋白 / 受傷後生存時間

Research Abstract

本研究では,大脳辺縁系・脳幹における急性ストレス反応を解明することを目的としている.各種中毒死例の脳組織内・脳脊髄液・血液における神経伝達物質であるドーパミン,セロトニン,アドレナリンとノルアドレナリンを測定し,マクロ的な急性ストレス反応を評価した.急性中毒死の36剖検例と非中毒死136例について血液,心膜液および脳脊髄液のアドレナリン(AD),ノルアドレナリン(NA),ドパミン(DA)およびセロトニン(5-HT)の測定値を比較検討した.中毒死では心内・末梢血,心膜液および特に脳脊髄液のDAの上昇が特徴的で,血中ADとNA,心膜液および脳脊髄液のNAと5-HTの上昇傾向がみられた.薬毒物別にみると,覚せい剤中毒では各部位のカテコラミン類が上昇傾向を示し,特に心内血と心膜液のADが高値であったが,血中濃度は症例によって大きく異なり,心膜液の5-HTは低値であった.向精神薬中毒では各部位のDAおよび心膜液のADは低値で,それ以外は覚せい剤と同様の上昇傾向がみられた.一酸化炭素中毒においては各部位のNAおよびDA並びに心膜液および脳脊髄液の5-HTは高値を示した.これらの濃度分布は各薬毒物の中毒作用を反映している可能性がある.
また,致死的外傷が生体に及ぼす病態を解明するため,剖検例336例におけるエリスローポエチン(EPO)およびC反応性蛋白(CRP)濃度を分析した.損傷死に関しては,急性死(生存時間1時間以内),亜急性死(同1〜1日以内)と遷延死(同1日〜1週間以内)に分けて検討した.その結果,右心内血EPOは,鈍器損傷例で,急死,亜急死および遷延死群の順に有意差が認められた.亜急死群では多発外傷死群が有意な高値を示した(p<0.01).鋭器損傷死群では,心臓・大動脈損傷群が腹部臓器・静脈損傷群よりも有意な高値を示した(p<0.001).一方,右心内血CRPは,鈍器損傷例の単独外傷群で,急死,亜急死および遷延死群の順に有意差が認められた(p<0.01),全例における右心内血EPOおよびCRPの測定値と受傷後経過時間との関係は,それぞれ有意な上昇が認められた.特に,鈍器損傷死の急死・亜急死例では,右心血EPOおよびCRPは多発外傷群で顕著な上昇を示した(r=0.8,p<0.001),以上から1日以内生存例の右心血EPO上昇の要因としては,外傷の重傷度と受傷後生存時間が最も影響したと考えられ,1日以上生存例では低酸素血症や感染症が影響したと考えられた.

Research Products

• [Journal Article] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. (2009)
  • Author(s): Quan L, Ishikawa T, Michiue T, Li DR, Zhao D, Yoshida C, Chen JH, Komatsu A, Azuma Y, Sakoda S, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • Peer Reviewed
• [Journal Article] Postmortem serum levels of pulmonary surfactant-associated proteins A and D with regard to the cause of death in medicolegal autopsy. (2009)
  • Author(s): Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Journal Title: Legal Med. (In press)
  • Peer Reviewed
• [Journal Article] Immunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. (2009)
  • Author(s): Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen JH, Wang Q, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • Peer Reviewed
• [Journal Article] Quantitative analysis of single-stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. (2009)
  • Author(s): Michiue T, Quan L, Ishikawa T, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • Peer Reviewed
• [Journal Article] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicolegal autopsy. (2009)
  • Author(s): Li DR, Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen JH, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Journal Title: Legal Med. (In press)
  • Peer Reviewed
• [Journal Article] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to lung weight in medicolegal autopsy cases. (2009)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatu A, Chen JH, Wang Q, Zhu BL, Maeda H.
  • Journal Title: Legal Med. (In press)
  • Peer Reviewed
• [Journal Article] Postmortem serum erythropoietin levels in establishing the cause of death and survival time at medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu BL, Ishikawa T, Michiue T, Zhao D, Li DR, Ogawa M, Maeda H.
  • Journal Title: Int J Legal Med. 122 Pages: 481-487
  • Peer Reviewed
• [Journal Article] Postmortem biochemistry and immunohistochemistry of adrenocort icotropic hormone with special regard to fatal hypothermia. (2008)
  • Author(s): Ishikawa T, Quan L, Li DR, Zhao D, Michiue T, Hamel M, Maeda H.
  • Journal Title: Forensic Sci Int. 179 Pages: 147-151
  • Peer Reviewed
• [Journal Article] Single-stranded DNA as an immunohistochemical marker of neuronal damage in human brain : an analysis of autopsy material with regard to the cause of death. (2008)
  • Author(s): Michiue T, Ishikawa T, Quan L, Li DR, Zhao D, Komatsu A, Zhu BL, Maeda H.
  • Journal Title: Forensic Sci Int. 178 Pages: 185-191
  • Peer Reviewed
• [Journal Article] Suicidal vehicle-assisted ligature strangulation resulting in complete decapitation : An autopsy report and a review of the literature. (2008)
  • Author(s): Zhao D, Ishikawa T, Quan L, Li DR, Michiue T, Maeda H.
  • Journal Title: Legal Med. 10 Pages: 310-315
  • Peer Reviewed
• [Journal Article] 片側性血胸に合併した対側性外傷性気胸による急死の1剖検例. (2008)
  • Author(s): 権力, 道上知美, 趙東, 李冬日, 石川隆紀, 前田均.
  • Journal Title: 法医病理 14 Pages: 22-28
  • Peer Reviewed
• [Presentation] Postmortem catecholamine levels in pericardial and cerebrospinal fluids with regard to the cause of death in medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Postmortem serum erythropoietin and C-reactive Protein levels as markers of survival time in injury deaths. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] lmmunohistochemistry of von Willebrand factor in the lungs with regard to the cause of death in forensic autopsy. (2008)
  • Author(s): Quan L, Ishikawa T, Zhao D, Michiue T, Yoshida C, Chen J-H, Wang Q, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Analyses of cardiac blood cells and serum proteins with regard to cause of death in forensic autopsy cases. (2008)
  • Author(s): Quan L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Komatsu A, Azuma Y, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Postmortem serum erythropoietin level with regard to cause of death and survival time in medicolegal autopsy. (2008)
  • Author(s): Ouan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Ogawa M, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Postmortem serum levels of pulmonary surfactant associated proteins A and D with regard to the cause of death in medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Postmortem serotonin levels in cerebrospinal and pericardial fluid with regard to the cause of death in medicolegal autopsy. (2008)
  • Author(s): Quan L, Zhu B-L, Ishikawa T, Michiue T, Zhao D, Yoshida C, Chen J-H, Wang Q, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Postmortem lung weight with regard to survival time. (2008)
  • Author(s): Chen J-H, Quan L, Ishikawa T, Michiue T, Wang Q, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Evaluation of postmortem calcium and magnesium levels in the pericardial fluid with regard to the cause of death in medicoleg al autopsy. (2008)
  • Author(s): Li D-R, Quan L, Zhu B-L, I shikawa T, Michiue T, Zhao D, Yoshida C, Komatsu A, Azuma Y, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Quantitative analysis of single stranded DNA immunoreactivity as a marker of neuronal apoptosis in hippocampus with regard to the causes of death in medicolegal autopsy. (2008)
  • Author(s): Michiue T, Quan L, Ishikawa T, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Evaluation of pulmonary GLUT1 and VEGF mRNA levels in relation to the lung weight in medicolegal autopsy cases. (2008)
  • Author(s): Zhao D, Ishikawa T, Quan L, Michiue T, Yoshida C, Komatsu A, Chen J-H, Wang Q, Zhu B-L, Maeda H.
  • Organizer: 7th International Symposium Advances in Legal Medicine
  • Place of Presentation: Osaka, Japan
  • Year and Date: 20080901-20080905
• [Presentation] Sudden death following a struggle during arrest by the police : four autopsy cases. (2008)
  • Author(s): Ishikawa T, Michiue T, Quan L, Zhu B-L, Maeda H.
  • Organizer: International Association of Forensic Sciences 18th Triennial Meeting
  • Place of Presentation: New Orleans, USA.
  • Year and Date: 20080721-20080725
• [Presentation] 薬毒物中毒死剖検例の血液・体液内カテコラミンおよびセロトニン濃度. (2008)
  • Author(s): 権力, 石川隆紀, 道上知美, 織谷茂樹, 小川雅史, 前田均.
  • Organizer: 第30回日本中毒学会総会・学術集会, 中毒研究2008 ; 21 : 439-440
  • Place of Presentation: 和歌山
  • Year and Date: 20080711-20080712
• [Presentation] 剖検例における血清エジスロポェチン濃度の分析. (2008)
  • Author(s): 権力, 朱宝利, 石川隆紀, 道上知美, 李冬日, 趙東, 吉田智美, 東洋子, 別所康守, 前田均
  • Organizer: 第92次日本法医学会総会
  • Place of Presentation: 長崎
  • Year and Date: 20080423-20080425