Analysis of relationship between exposure to asbestos on immunocompetent cells and mechanisms of asbestos-induced tumor progression

2008 Fiscal Year Final Research Report

• Project/Area Number: 19890253
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Kawasaki Medical School
• Principal Investigator: SHUKO Murakami Kawasaki Medical School, 衛生学, 助教 (50454848)
• Project Period (FY): 2007 – 2008
• Keywords: アスベスト / 細胞増殖 / 転写因子 / アポトーシス

Research Abstract

The establishment of in vitro model using cell line for long-term and low-dose exposure model to asbestos on immunocompetent cells and immunological assessment of cases exposed silica, and asbestos were performed. Although increased production of IL-10, which is known as immune-suppressive cytokine, and decreased production of IFNγ, which is immune-activating cytokine, were observed by in vito model, the serum concentration of various cytokines from silicosis and asbestos-exposed patients did not show any apparent difference among these groups except high IL-6 (probably produced by tumor cells) in mesothelioma patients.

Research Products

• [Journal Article] Cytokine alteration and speculated immunological pathophysiology in silicosis and asbestos-related diseases (2009)
  • Author(s): Murakami S, Nishimura Y, Maeda M, Kumagai N, Hayashi H, Chen Y, Kusaka K, Kishimoto T, Otsuki t.
  • Journal Title: Environ Health Prev Med 14 (4) Pages: 216-222
• [Journal Article] Impairment in cytotoxicity and expression of NK-cell activating receptors on human NK cells following exposure to asbestos fibers (2009)
  • Author(s): Nishimura Y, Miura Y, Maeda M, Kumagai N, Murakami S, Hayashi H, Fukuoka K, Nakano T, Otsuki T.
  • Journal Title: Int J Imminopathol Pharmacol 22 (3) Pages: 579-90
• [Journal Article] Soluble interleukin-2 receptor as an indicator of immunological disturbance found in silicosis patients (2009)
  • Author(s): Hayashi H, Maeda M, Murakami S, Kumagai N, Chen Y, Hatayama T, Katoh M, Miyahara N, Yamamoto S, Yoshida Y, Nishimura Y, Kusaka M, Fujimoto W, Otsuki T.
  • Journal Title: Int J Imminopathol Pharmacol 22 (1) Pages: 53-62
• [Journal Article] Is soluble CD40 ligand an indicator of immunopathological disturbance in silicosis patients? (2009)
  • Author(s): Hayashi H, Nishimura Y, Maeda M, Murakami S, Kumagai N, ChenY, Kusaka M, Uragami K, Fujimoto W, Otsuki T.
  • Journal Title: Kawasaki Med J 35 (2) Pages: 129-138
• [Journal Article] Immunological alterations found in mesothelioma patients and their experimental evidences (2008)
  • Author(s): Miura Y, Nishimura Y, Maeda M, Murakami S, Hayashi H, Fukuoka K, Kishimoto T, Nakano T, Otsuki T.
  • Journal Title: Environ Health Prev Med 13 Pages: 55-59
• [Journal Article] Immunological changes in mesothelioma patients and their experimental detection (2008)
  • Author(s): Maeda M, Miura Y, Nishimura Y, Murakami S, Hayashi H, Kumagai N, Hatayama T, Katoh M, Miyahara N, Yamamoto S, Fukuoka K, Kishimoto T, Nakano T, Otsuki T.
  • Journal Title: Clin Med : Circ, Resp Pulm Med 2 Pages: 11-17
• [Journal Article] Lifestyle and T-helper 1 and 2 related cytokines in healthy volunteers (2008)
  • Author(s): Miura Y, Ishibashi T, Tatsukawa T, Maeda M, Murakami S, Nishimura Y, Kumagai N, hayashi H, Ying C, Hyodo F, Kojima S, Fujii M, Morimoto K.
  • Journal Title: Kawasaki Medical Journal 34 (3) Pages: 171-177
• [Journal Article] アスベスト関連疾患の発症機構-悪性中皮腫を中心として- (2008)
  • Author(s): 西村泰光, 前田恵, 村上周子, 熊谷直子, 林宏明, 大槻剛巳
  • Journal Title: Surgery Frontier 15 (2) Pages: 8-13
• [Journal Article] 珪肺症例CD4+25+分画における活性化T細胞の混入と制御性T細胞早期細胞死の可能性 (2008)
  • Author(s): 林宏明, 三浦由恵, 前田恵, 村上周子, 熊谷直子, 西村泰光, 草加勝康, 大槻剛巳
  • Journal Title: 日本職業・環境アレルギー学会雑誌 15 (2) Pages: 43-50
• [Journal Article] 大気環境汚染による肺疾患 (2008)
  • Author(s): 村上周子, 前田恵, 林宏明, 西村泰光, 大槻剛巳
  • Journal Title: 日本臨床別冊 呼吸器症候群 (I)-その他の呼吸器疾患を含めて- Pages: 593-596
• [Journal Article] 新しい環境中物質, 繊維・粒子状物質による生体影響へのアプローチ-シンポジウム「日本臨床環境医学の近未来」によせて- (2008)
  • Author(s): 熊谷直子, 西村泰光, 前田恵, 村上周子, 林宏明, 陳瑩, 平野靖史郎, 大槻剛巳
  • Journal Title: 臨床環境医学 17 Pages: 83-94
• [Journal Article] Immunological effects of silica and asbestos (2007)
  • Author(s): Otsuki T, Maeda M, Murakami S, Hayashi H, Miura Y, Kusaka M, Nakano T, Fukuoka K, Kishimoto T, Hyodoh F, Ueki A, Nishimura Y.
  • Journal Title: Cell Mol Immnol 4 (4) Pages: 261
• [Journal Article] 珪酸・アスベストの免疫系への影響 (2007)
  • Author(s): 大槻剛巳, 前田恵, 三浦由恵, 兵藤文則, 植木絢子, 村上周子, 林宏明, 勝山博信, 冨田正文, 西村泰光
  • Journal Title: 日本職業災害医学会会誌 55 (3) Pages: 113-120
• [Presentation] Suppressive effect of asbestos-exposure on cytotoxicity of human NK cells, and the possibility of NKp46 as a marker to monitor immune status in people exposed to asbestos (2009)
  • Author(s): Nishimura Y, Maeda M, Kumagai N, Murakami S, Hayashi H, Kishimoto T, Fukuoka K, Nakano T, Otsuki T.
  • Organizer: 第2回日中石綿シンポジウム
  • Place of Presentation: New Century Zhijiang Resort Hangzhou, Hangzhou, China
  • Year and Date: 20090417-20090418
• [Presentation] Immunotoxicological effects of asbestos on human T cells (2009)
  • Author(s): Otsuki T, Maeda M, Murakami S, Miura Y, Kumagai N, Chen Y, Hayashi H, Nakano T, Fukuoka K, Kishimoto T, Nishimura Y.
  • Organizer: The 48th Society of Toxicology (USA)
  • Place of Presentation: Baltimore Convention Center in Baltimore, Maryland
  • Year and Date: 20090305-20090319
• [Presentation] Immunological changes in mesothelioma patients and their experimental detection (2008)
  • Author(s): Otsuki T, Maeda M, Murakami S, Hayashi H, Kumagai N, Miura Y, Chen Y, Kuribayashi K, Fukuoka K, Kishimoto T, Nakano T, Nishimura Y.
  • Organizer: Inetrnational Mesothelioma Interest Group
  • Place of Presentation: Congress 2008. Amsterdam
  • Year and Date: 20080925-20080927
• [Presentation] Impairment in cytotoxicity and expression of NK-cell activating receptors on human NK cells caused by exposure to asbestos fibers (2008)
  • Author(s): Nishimura Y, Maeda M, Murakami S, Hayashi H, Miura Y, Fukuoka K, Nakano T, Otsuki T.
  • Organizer: Inetrnational Mesothelioma Interest Group. Congress 2008
  • Place of Presentation: Amsterdam
  • Year and Date: 20080925-20080927
• [Presentation] Down-regulation of chemokine receptor CXCR3 in peripheral T lymphocytes from patients with asbestos-related disease (2008)
  • Author(s): Maeda M, Nishimura Y, Murakami S, Kumagai N, Hayashi H, Ying C, Miura Y, Nakano T, Kishimoto T, Otsuki T.
  • Organizer: Inetrnational Mesothelioma Interest Group
  • Place of Presentation: Congress 2008. Amsterdam
  • Year and Date: 20080925-20080927
• [Presentation] Reduced function of the peripheral CD4+25+ fraction in silicosis patients exposed to environmental factors for SSc (2008)
  • Author(s): Hayashi H, Miura Y, Maeda M, Murakami S, Nishimura Y, Kusaka M, Fujimoto W, Otsuki T.
  • Organizer: The 5th International Investigative Dermatology
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 20080514-20080517
• [Presentation] ヒトポリクローナルT細胞株のアスベスト誘導アポトーシス抵抗性亜株における転写因子に関する検討 (2008)
  • Author(s): 村上周子, 前田恵, 三浦由恵, 西村泰光, 熊谷直子, 林宏明, 大槻剛巳
  • Organizer: 第78回日本衛生学会総会
  • Place of Presentation: 熊本市民会館
  • Year and Date: 20080328-20080331
• [Presentation] Immunological effects of asbe4stos (2007)
  • Author(s): Otsuki T, Maeda M, Miura Y, Murakami S, Hayashi H, Nishimura Y, Kuribayashi K, Fukuoka K, Nakano T.
  • Organizer: 66th Annual Meetingof the Japanese Cancer Association
  • Place of Presentation: Pacifica Yokohama
  • Year and Date: 20071003-20071005
• [Book] Immunological effects of Asbestos(Asbestos : Risks, Environment and Impact. Eds : Antonio Soto and Gael Salazar, ISBN 978-1-60692-053-4 (C) 2009) (2009)
  • Author(s): Otsuki T, Maeda M, Miura Y, Hayashi H, Murakami S, Kumagai N, Nishimura Y.
  • Publisher: Nova Science Publishers, Inc
• [Book] Establishment of Negatively-charged Indoor Air Conditions and their Biological Effects(Buildings and the Environment. Authors/Editors : Jonas Nemecek and Patrik Schulz, Pub. Date : 2009 4th Quarter ISBN : 978-1-60876-128-9 (C) 2009) (2009)
  • Author(s): Otsuki T, Takahashi K, Mase A, Kawado T, Kotani M, Nishimura Y, Maeda M, Murakami S, Kumagai N, Hayashi H, Chne Y, Miura Y, Shirahama T, Yoshimatsu M, Morimoto K.
  • Publisher: Nova Science Publishers, Inc
• [Remarks] 川崎医科大学衛生学のHPに研究概要と業績を紹介している。
  • URL: http://www.kawasaki-m.ac.jp/hygiene/