2019 Fiscal Year Annual Research Report
Next Generation Implantable Nanosensor Technology for Monitoring Single Cell Metabolites
| Project/Area Number |
19F19076
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| Research Institution | Tokai University |
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Principal Investigator |
槌谷 和義 東海大学, 工学部, 教授 (50399086)
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| Co-Investigator(Kenkyū-buntansha) |
MANI GANESH KUMAR 東海大学, 工学部, 外国人特別研究員
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| Project Period (FY) |
2019-07-24 – 2021-03-31
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| Keywords | Microneedle / Sensor / Single cell / Cell metabolites / Point of care devices |
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| Outline of Annual Research Achievements |
1. To fabricate successful masking, a new masking technique was designed and tested successfully. Successfully masked the needle tip using low temperature melted metal at the micro level.
2. Needle tip diameter investigation was done by making various sets of fabrication technique and optimized. Multiple needles fabrication was done at the same time and the investigation is been initiated.
3. Cytotoxicity experiments were conducted using HepG2 and A549 cells. The results proved that the cell growth was not affected by Ag/AgIO3 and Sb/Sb2O3 electrodes.
4. Needle mask removal strategy process is under processing by removing with hot water and studying its elemental composition analysis.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
1 Needle electrode masking creates lot of failures in fabrication of pH sensor. Previously needle electrode was masked with polymer-based resin. Hence started searching for new method to mask at micro level.
2 Needle masking difficulties is tried to overcome by employing new low temperature melting metal (above 100oC). Hence, usually after all the sputtering process, needle will be dipped into hot water and then taken for analysis.
3 Using the above technique, somewhat the needle masking was done near perfectly at the micro level.
4 The deposition of Sb2O3 and Ag/AgIO3 was done successfully using the new masking technique.
5 Cytotoxicity experiments was conducted with biological cells and found that the microneedle electrode is fully biocompatible.
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| Strategy for Future Research Activity |
1 More analytical characterizations are planned to investigate about the reactions between low temperature metal and sensor electrodes
2 Biomarker such as glucose/methylglyoxal testing is planned to do with the fabricated microneedle
3 Electrode material evaluation of structural and morphological properties of each electrode materials by FESEM, XRD and XPS.
4 To improve the selectivity of the sensor, the interference studies will be carried out using uric acid, lactate and dopamine.
5 Cytotoxicity experiments were planned to conduct with human skin cells and in-vivo testing with real animal models
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