2019 Fiscal Year Annual Research Report
Mechanisms of flattening growth regulation in unifacial leaves by PRS genes
| Project/Area Number |
19F19085
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| Research Institution | The University of Tokyo |
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Principal Investigator |
塚谷 裕一 東京大学, 大学院理学系研究科(理学部), 教授 (90260512)
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| Co-Investigator(Kenkyū-buntansha) |
YIN XIAOFENG 東京大学, 理学(系)研究科(研究院), 外国人特別研究員
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| Project Period (FY) |
2019-04-25 – 2021-03-31
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| Keywords | Juncus / leaf development / mold / polarity / PRESSED FLOWERb / unifacial leaf |
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| Outline of Annual Research Achievements |
In the last year, the expression pattern of key genes including PRSb was checked, using in situ hybridization, in mold-treated samples of J. prismatocarpus. Preliminary results revealed that PRSb expression is lost when the shape of the leaf is changed from flattened to round. On the other hand, the expression pattern of another gene, KN1 (functions in the shoot apical meristem), was not changed. These results confirm the original hypothesis that the expression of PRSb (but not other genes) depends on the leaf shape instead of the juxtaposition of the adaxial and the abaxial polarity.
More importantly, after a few trials, the shape change of J. wallichianus from round to flattened (elongated) has been achieved. The size of the mold is 1 mm × 2 mm. After 2 weeks of free in vitro growth and 4 weeks of mold growth on vermiculite, about 80% of the samples could be successfully changed.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The progress so far is as expected. The most challenging part of the research, inducing leaf shape change from flattened to round in J. prismatocarpus, and from round to flattened in J. wallichianus, has been achieved. Genes to be investigated have already been cloned. Expression patterns of these genes in J. prismatocarpus (both the original shape and the changed shape) have been checked using in situ hybridization. qRT-PCR of these genes and the expression patterns of these genes in J. wallichianus (both the original shape and the changed shape) is currently under preparation.
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| Strategy for Future Research Activity |
1.Using qRT-PCR to examine how the expression of PRSb is related to different leaf flatness. Also using qRT-PCR to examine how the expression of other genes (DROOPING LEAF, KNOTTED1) is affected, to understand how the mold treatment itself would affect gene expression in general.
2.Check how altered PRSb expression would affect cell division activities in the presumed marginal areas, using pulse-chase EdU assay (Yin and Tsukaya, 2016. New Phytologist. 211: 1462-1469; Yin and Tsukaya, 2019. New Phytologist. 222: 1101-1111).
3.Check the expression pattern of PRSb, DL, and KN1 in mold-treated J. wallichianus, which has been successfully changed its leaf blade shape from round to flattened in the last year, using in situ hybridization and qRT-PCR.
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