2020 Fiscal Year Annual Research Report
音響ＧＰＳロガーを用いた，人為的景観環境下の野生コウモリの移動生態に関する研究
| Project/Area Number |
19F19090
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| Research Institution | Doshisha University |
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Principal Investigator |
飛龍 志津子 同志社大学, 生命医科学部, 教授 (70449510)
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| Co-Investigator(Kenkyū-buntansha) |
HEIM OLGA 同志社大学, 生命医科学部, 外国人特別研究員
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| Project Period (FY) |
2019-10-11 – 2022-03-31
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| Keywords | Movement behavior / Diet analysis / Neural Networks |
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| Outline of Annual Research Achievements |
During the academic year of 2020/2021, I joined a field trip to Hokkaido and to the Fukui prefecture in order to capture and tag Japanese horseshoe bats and birdlike noctules and to collect fecal as well as fur samples from these two bat species. In order to further investigate the type of insects that are available as prey, also insects from different habitat types have been captured. In addition, to investigate the health status of birdlike noctules, we collected also blood samples. During these trips, a lot of data could be collected.
After these trips, I continued to work on preparing DNA-samples obtained from fecal samples for Next Generation Sequencing. During winter 2020, these samples have been sequenced. In early 2021 these data were bioinformatically analyzed.
The available GPS-data from bats that were tracked from the year 2015 to 2020 is under analysis. A majority of statistical tests on GPS-data from Japanese horseshoe bats have been finished and presented at the International Berlin Bat Meeting in March 2021. The corresponding manuscript is in preparation.
Due to the relatively limited field-work caused by the Corona-virus pandemic, we started a new project that does not require collecting data in the field. We recorded echolocation calls from Miniopterus fuliginosus bats that are kept at the laboratory, in order to investigate whether it is possible to discriminate echolocation calls between individuals using neural networks. We collected a sufficiently large data base of around 1000 calls for 7 individuals and preliminary analyses were conducted.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Regarding the research on bat movement behavior of wild bats, we have sufficient data for the horseshoe bats - especially pregnant females. There is also a good amount of GPS-data for birdlike notcules - especially for post-lactating females, so that it should be possible to publish that data in the near future, too.
Regarding the analysis of wild bat’s diet composition, we are also progressing well, since the first set of samples has been sequenced and bioinformatically analyzed. And another big set of DNA-samples including whole insects is waiting to be processed.
In addition to the original research plan, we were able to add a few new facets in order to compare measures of the health status such as information on the immune system and chronic stress, based on blood samples.
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| Strategy for Future Research Activity |
My plan for the next few months is to submit the manuscript on the movement behavior of Japanese greater horseshoe bats to a high-ranking journal. Furthermore, I plan to prepare the new set of DNA-samples for the next generation sequencing, so that our collaborator Dr. Eero Vesterinen in Finland can analyze the data bioinformatically before sending them back to us for a statistical analysis. Parallel to these two main goals, I would like to participate in fieldwork trips to the Fukui prefecture and to Hokkaido, if possible. Furthermore, I would like to prepare a draft manuscript based on the analyzed DNA-samples.
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Research Products
(2 results)
