2020 Fiscal Year Annual Research Report
Influence of non-vascular cells in accelerated coronary aging in diabetes
| Project/Area Number |
19F19708
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Pearson James 国立研究開発法人国立循環器病研究センター, 研究所, 部長 (30261390)
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| Co-Investigator(Kenkyū-buntansha) |
NGO JENNIFER 国立研究開発法人国立循環器病研究センター, 研究所, 外国人特別研究員
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| Project Period (FY) |
2019-07-24 – 2021-03-31
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| Keywords | coronary vessels / diabetes / ageing / oxidative stress / vasodilators |
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| Outline of Annual Research Achievements |
Metabolome analyses of myocardium from senescence accelerated mice (SAMP8) and resistance mice (SAMR1) on a high fat diet (HFD) revealed that in SAMP8 mice glycolysis was inhibited and abnormal purine metabolism increased xanthine oxidase (XO) activation and ROS generation relative to SAMR1 mice. While antioxidant GSH was upregulated and L-arginine availability was maintained in the HFD SAMP8 mouse hearts we found that eNOS phosphorylation at Ser1177 was downregulated in SAMP8 and upregulated in SAMR1 on the HFD. Notably, this resulted in reduced coronary microvessel perfusion (microangiography), but not macrovessels in the HFD SAMP8 mice. Importantly, high intensity exercise training for 8wks restored microvessel perfusion in the HFD SAMP8 through an increased NO contribution.
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| Research Progress Status |
令和2年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和2年度が最終年度であるため、記入しない。
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