2020 Fiscal Year Annual Research Report
非天然型アミノ酸生産のためのＰＬＰ依存性アミノ基転移酵素の改変
| Project/Area Number |
19F19764
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
LAURINO Paola 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 准教授 (90812256)
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| Co-Investigator(Kenkyū-buntansha) |
DINDO MIRCO 沖縄科学技術大学院大学, タンパク質工学・進化ユニット, 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Keywords | Disordered regions / AGT / protocells / Liquid phase separation |
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| Outline of Annual Research Achievements |
In this year we have mainly worked with the alanine:glyoxylate aminotransferase, because we discovered some intrinsically disordered regions on the protein surface. In detail we have performed several investigation on these regions from a bioinformatic (molecular dynamics), biochemical (library screening and production and mutants purification as well) and cellular point of view. In the meantime we are also working in the preparation of a new system to study enzymes behavior, generating enzymatically active protocells using the liquid-liquid phase separation.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The paper on the disordered regions of AGT is ready for submission and the other one of the enzymatically active protocells as well. We are just working on the final details before submission.
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| Strategy for Future Research Activity |
The project about DPAT did not give the expected results, we are still optimizing the assay and the engineering part of the protein. We are also currently working with the enzymatically active protocells , in detail we are studying the effects of enzymatic activity on the protocells environment.
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