2021 Fiscal Year Annual Research Report
| Project/Area Number |
19F19782
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
白須 賢 国立研究開発法人理化学研究所, 環境資源科学研究センター, グループディレクター (20425630)
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| Co-Investigator(Kenkyū-buntansha) |
FISHMAN MAX 国立研究開発法人理化学研究所, 環境資源科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2019-11-08 – 2022-03-31
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| Keywords | Parasiticplants / Calciumsignaling / phosphoproteomics / DMBQ / hustorium |
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| Outline of Annual Research Achievements |
During 2021, I identified endogenous Phtheirospermum japonicum peptide hormones that are induced upon treatment with the haustorium inducing compound (HIF) 2,6 dimethoxybenzoquinone (DMBQ). I found that treatment of P. japonicum with an endogenous peptide induced haustorium formation without the treatment of an HIFs and induced expression of the known haustorium formation marker gene PjYUC3. Further investigation revealed that there are three additional putative peptide hormones in the P. japonicum genome and six putative peptide receptors. Further investigation found that only one of those three peptides is up-regulated following DMBQ treatment while none of the receptors are up-regulated following DMBQ treatment. I further characterized the expression of the peptides and receptors using transformed P. japonicum hairy roots expressing reporter gene constructs. I found that only one of the four peptides and potentially three of the receptors are expressed in the haustorium following DMBQ treatment. Lastly, PLETHORA (PLT) transcription factors are stabilized post-translationally by the peptides. Using hairy root transformation, I found that DMBQ treatment resulted in movement of a PjPLT protein up the root and that this PjPLT localized in the haustorium.
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| Research Progress Status |
令和3年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和3年度が最終年度であるため、記入しない。
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