2021 Fiscal Year Final Research Report
Rational control of gene expressions by chemically regulating topologies of nucleic acid structures
| Project/Area Number |
19H00928
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Medium-sized Section 37:Biomolecular chemistry and related fields
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| Research Institution | Konan University |
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Principal Investigator |
Sugimoto Naoki 甲南大学, 先端生命工学研究所, 教授 (60206430)
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| Co-Investigator(Kenkyū-buntansha) |
建石 寿枝 甲南大学, 先端生命工学研究所, 准教授 (20593495)
高橋 俊太郎 甲南大学, 先端生命工学研究所, 准教授 (40456257)
遠藤 玉樹 甲南大学, 先端生命工学研究所, 准教授 (90550236)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 分子環境 / 核酸構造 / トポロジー / 遺伝子発現制御 / 人工分子 |
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| Outline of Final Research Achievements |
In this research project, gene regulation mechanisms based on nucleic acid structures, whose topological properties dynamically fluctuate depending on the molecular environment in cells, were investigated through quantitative analyses of their structures and stabilities. Physicochemical factors that affect the structures and stabilities of nucleic acids have been elucidated under molecular crowding conditions. Correlations between the nucleic acid structures and their functions to modulate gene expressions were also investigated. Furthermore, the construction of artificial molecules was demonstrated to apply to the regulation of nucleic acid functions based on the modulation of the dynamics and stabilities of their structures. The results of this research project are expected to provide great advances in not only the academic chemistry and biology of nucleic acids but also in a wide range of applied fields such as therapeutic, diagnostic, and medicinal applications.
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| Free Research Field |
核酸化学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題では、分子クラウディング環境を中心に、核酸の分子内、分子間での相互作用を定量的に解析してエネルギーレベルのデータベースを蓄積した。本研究で蓄積したデータベースは、核酸トポロジーの細胞内における分子挙動の予測に有用であり、基礎学術的に非常に価値の高いものである。核酸分子の異常な構造変化は、癌や神経変性疾患、生活習慣病などの原因となる可能性も指摘されつつある。それ故、研究成果として、人工分子を用いての核酸機能の化学的な制御が可能であることを実証したことで、医療、診断、創薬など、実社会に貢献する幅広い応用分野への研究展開が期待される。
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