2022 Fiscal Year Final Research Report
Development of E. coli translational system
Project/Area Number |
19H00936
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
|
Research Institution | Osaka University (2022) National Institute of Advanced Industrial Science and Technology (2019-2021) |
Principal Investigator |
Miyazaki Kentaro 大阪大学, 生物工学国際交流センター, 特任教授(常勤) (60344123)
|
Project Period (FY) |
2019-04-01 – 2023-03-31
|
Keywords | リボソーム / ホーミングエンドヌクレアーゼ |
Outline of Final Research Achievements |
To innovate the translational function of E. coli, heterologous 23S rRNA gene was PCR amplified from the environmental metagenomes. The amplicon was incorporated into an expression vector, and E. coli strains deficient in the 23S rRNA gene were screened by means of genetic complementation as, and mutants with faster growth rates were accumulated. The 23S rRNA gene sequence was inserted at a homologous site with an intron containing a homing endonuclease (HE) in the 23S rRNA gene of Rhodothermus marinus, which was discovered previously, but failed to replace the E. coli 23S rRNA genes in the genome.
|
Free Research Field |
生物工学
|
Academic Significance and Societal Importance of the Research Achievements |
バクテリアイントロンはほとんど報告がないが、本研究提案の基礎となる23S rRNA遺伝子中のホーミングエンドヌクレアーゼを含むイントロンの発見も極めてユニークであり、高い意義がある。大腸菌のもつ23S rRNAより高性能の23S rRNAのスクリーニングを行い、宿主の増殖を良化する遺伝子の獲得には成功し、大腸菌リボソームに改良の余地があることが示されたことは高い意義がある。
|