2023 Fiscal Year Final Research Report

Regulatory mechanisms of seminal proteins on uterine function and its application to improvement of fertility in cows

Research Project

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Project/Area Number	19H00964
Research Category	Grant-in-Aid for Scientific Research (A)
Allocation Type	Single-year Grants
Section	一般
Review Section	Medium-sized Section 42:Veterinary medical science, animal science, and related fields
Research Institution	Hokkaido University
Principal Investigator	Katagiri Seiji 北海道大学, 獣医学研究院, 教授 (00292061)
Co-Investigator(Kenkyū-buntansha)	柳川洋二郎北海道大学, 獣医学研究院, 准教授 (20609656) 杉浦智親酪農学園大学, 獣医学群, 助教 (40828258) 奥山みなみ大分大学, 医学部, 助教 (50756781) 平山博樹東京農業大学, 生物産業学部, 教授 (60390861) 永野昌志北里大学, 獣医学部, 教授 (70312402) 田上貴祥北海道大学, 農学研究院, 助教 (70709849)
Project Period (FY)	2019-04-01 – 2024-03-31
Keywords	低受胎 / 乳牛 / 子宮 / 上皮成長因子 / Toll様受容体 / レプチン受容体
Outline of Final Research Achievements	We investigated the role of the endocrine and immune systems in abnormal EGF concentration in the uterus of dairy cows. First, we investigated the effects of IGF-I and leptin, which link reproductive function and nutritional status, and found that there was no difference in blood concentrations of either of them between abnormal and normal cows. However, the expression of leptin receptors was found to be increased in abnormal cows, and it was also found to be associated with resistance to treatment. Next, when we compared the expression of cytokines in the blood and uterus before and after treatment, we found that there was a transient increase in the expression of inflammatory cytokines after treatment, but no relationship was seen with treatment outcomes. However, it was suggested that increased expression of TolI-like receptors, which are associated with innate immunity, is associated with resistance to treatment.
Free Research Field	臨床繁殖学
Academic Significance and Societal Importance of the Research Achievements	子宮でのEGF濃度異常と免疫系、とくにToll様受容体を介した自然免疫系の関連が示されたことにより、従来から試みられてきた内分泌系を標的とした治療法に加えて、Toll様受容体を標的とする新たな予防及び治療法の開発に道が拓けた。低受胎牛の問題は、学術的にはその対策に手詰まり感が感じられていた問題であり、この発見は関連する栄養学、管理学及び臨床獣医学の各分野での研究の活性化につながることが期待される。