2021 Fiscal Year Final Research Report

Active control and simulator of neurons in regenerative transplantation treatment with the aid of environment control utilizing three-dimensional dynamic stimulations

Research Project

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Project/Area Number	19H02089
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 20010:Mechanics and mechatronics-related
Research Institution	Yamagata University
Principal Investigator	Kosawada Tadashi 山形大学, 大学院理工学研究科, 教授 (10143083)
Co-Investigator(Kenkyū-buntansha)	馮忠剛山形大学, 大学院理工学研究科, 准教授 (10332545)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	iPS細胞 / ニューロン分化・成長誘導制御 / ３次元ゲル包埋培養 / ３次元ニューロンネットワーク / in vitro移植シミュレータ / 超小型３次元振動ステージ / ３次元動的力学刺激環境 / 移植性促進法
Outline of Final Research Achievements	Not like a conventional approach but based on the principle of mechanics, a novel small sized piezoelectric 3-D vibration table is developed to impose dynamic stimulations on cultured living cells in order to support regenerative medicine. Together with scaffold utilizing gel-embedded 3-D culture, the device enables us to enforce 3-D micro dynamic stimulations upon the cultured neuronal networks. Appropriately soft but structurally stable scaffold is realized by optimization of collagen-gel concentration, which provides an effective method for the gel-embedded 3-D culture of neurons differentiated from iPS cells. Enforcing systematic 3-D dynamic stimulations upon gel-embedded neuronal networks by the developed 3-D vibration table, principle usefulness of the present study as a 3-D manipulation and active control method of neuronal networking as well as simulation system for regenerative transplantation medicine has been confirmed.
Free Research Field	医用生体工学，機械力学
Academic Significance and Societal Importance of the Research Achievements	本研究では，iPS細胞を用いた再生治療に貢献するため，生きている培養細胞に対し従来の化学的誘導因子に加え，３次元の動的力学刺激を自在に付加し得る圧電駆動超小型３次元振動ステージを開発した。ゲル包埋３次元培養法と合わせ，これまでとは全く異なる力学的原理に基づく３次元ニューロンネットワークの創生・誘導制御および再生移植シミュレーションを可能とする革新的システムを開発した。今後の更なる研究により，脊髄損傷やパーキンソン病等神経系難病に対する再生移植治療の実現を支援・推進するデバイス，スキルに展開し得る可能性を提示した。