2021 Fiscal Year Final Research Report
Multi-scale cell analysis system using opto-chemo robots
| Project/Area Number |
19H02096
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 20010:Mechanics and mechatronics-related
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | マイクロ・ナノメカトロニクス / 光環境センサ / 光ピンセット / 細胞解析 |
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| Outline of Final Research Achievements |
In this research project, we developed two types of devices for measuring the physiological state of cells on a multi-scale from a single cell level to a group dell level. (1) a capsule-type opto-chemo robot that is driven, measured, and stimulated by using light with different wavelengths, which is fabricated from functional particles with photo-responsive reagents, and (2) a chip-type opto-chemo robot that is driven, measured, and stimulated by using light with different wavelengths. (2) a chip-type opto-chemo robot that is driven, measured, and stimulated using light of different wavelengths.
As achievements, 1 um-diameter capsule-type optical sensor for intracellular measurement that has temperature measurement function, transportation using 1064 nm laser, and heating function using 808 nm laser were fabricated. And a chip-type opto-chemo robot for on-chip pH measurement with reference function for absolute measurement of pH has been successfully fabricated.
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| Free Research Field |
マイクロ・ナノメカトロニク
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| Academic Significance and Societal Importance of the Research Achievements |
本研究課題の実施期間に得られた，細胞内および培養環境の環境情報を精密に計測する技術は，工学的アプローチを基盤とし，生物化学において新しい知見を提供するための光化学・分子化学的アプローチを複合的に用いることを特色とした新規な微細操作・計測技術の開拓が期待される成果である．また，本成果は，例えばウイルス感染細胞におけるウイルス増殖過程の細胞の状態計測を行うことで，ウイルスが増えやすい，もしくは増えにくいように細胞機能を誘導する技術にブレークスルーを起こすことが期待でき，新たな感染症対策を開拓する可能性を有していると考える．
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