Dynamic Processes of Bacterial Stress Response: Development of Novel Bacterial Identification Method Inspired by Electronics

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02196
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 21060:Electron device and electronic equipment-related
• Research Institution: Toyohashi University of Technology
• Principal Investigator: Ishii Hiromu 豊橋技術科学大学, 研究推進アドミニストレーションセンター, 特任教授 (20506175)
• Co-Investigator(Kenkyū-buntansha): 齋藤 光正 産業医科大学, 医学部, 教授 (00315087) ; 町田 克之 東京工業大学, 科学技術創成研究院, 特任教授 (90597676)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: Bio-MEMS / 細菌 / フォトゲート / レジオネラ属菌 / 反応化学

Outline of Final Research Achievements

Legionella species, one of the pathogenic bacteria that are endemic to the environment such as biofilms, was used as a model bacterium to elucidate the dynamic process of bacteria. The dynamic process of the stress response of Legionella species to UV light was described by the reaction kinetics as the decrease and increase of intracellular fluorescent substances. We also developed a bacterial sensor system by hybridizing a photogate-type bacterial sensor with a MEMS microfluidic. That is, we developed a compact and portable bacterial sensor system of about 3.5x13 cm2 by mounting a photogate-type bacterial sensor and a MEMS microfluidic in a housing fabricated by a 3D printer. Using this bacterial sensor system, we observed the dynamic process of fluorescence of Legionella bacteria and succeeded in identifying Legionella species.

Free Research Field

集積化MEMS

Academic Significance and Societal Importance of the Research Achievements

バイオフィルムなどの環境に常在する病原細菌の一つであるレジオネラ属菌を細菌の動的過程解明のモデル細菌とし、このレジオネラ属菌の紫外光や化学物質に対するストレス応答の動的過程を細胞内の蛍光物質の減少、増加過程として反応化学的に記述した。さらにフォトゲート型細菌センサチップをMEMS流路とハイブリッド化した小型可搬な細菌センサシステム構築をした。これにより、培養期間を要することなく医療現場や高齢者施設で即時的に病原細菌の検知を可能とし、感染のアウトブレイク前に警告を発する可能性を示すことができる。