2021 Fiscal Year Final Research Report

Cell metabolic activity via non-vacuum ion implantation utilizing an enhanced electric field at a sharpened glass tip

Research Project

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Project/Area Number	19H02432
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Review Section	Basic Section 26020:Inorganic materials and properties-related
Research Institution	Nagoya Institute of Technology
Principal Investigator	Daiko Yusuke 名古屋工業大学, 工学(系)研究科(研究院), 准教授 (70514404)
Co-Investigator(Kenkyū-buntansha)	豊田紀章兵庫県立大学, 工学研究科, 教授 (00382276) 篠崎健二国立研究開発法人産業技術総合研究所, 材料・化学領域, 主任研究員 (10723489) 小幡亜希子名古屋工業大学, 工学(系)研究科(研究院), 准教授 (40402656) 本間剛長岡技術科学大学, 工学研究科, 准教授 (70447647)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	ガラス / イオン伝導 / イオン放出 / 大気圧プラズマ / 細胞応答 / マクロファージ
Outline of Final Research Achievements	Under atmospheric pressure conditions, in addition to field ion emission from electrolytes, atmospheric discharges and other discharge events occur. By combining a quartz crystal microbalance and electrochemical measurements, both the released ion current and mass were measured simultaneously. By using silver ion-conductive glass with a thermal expansion coefficient close to that of the silver electrode, the lifetime of silver ion release under atmospheric pressure was extended from about several minutes to about 4 hours, and it was confirmed that there was no release other than silver ions and that silver ions were supplied from the silver electrode to the glass. Direct ion irradiation of living cells was performed, and it was observed that the cell behavior changed depending on the amount, energy, and type of irradiated ion species.
Free Research Field	無機材料
Academic Significance and Societal Importance of the Research Achievements	生細胞(NIH3T3)にプロトンもしくはAg+イオンを直接照射すると，未照射条件と比較して1~2ピコモル程度のごく少量イオンを照射したのち培養すると細胞がより増殖するのに対して，照射量を増やすと逆に増殖能が低下して細胞死を招くことを高い再現性で確認している。増殖能の向上は外傷の早期治癒，また細胞死の誘導は不良細胞やかゆみなどの原因菌の死滅などにつながる知見であり，この細胞増減を決定するイオン照射量のしきい値を明らかにすることで，イオン照射の応用が広がると考えられる。