Development of biofilm formation control technology using colloidal technique

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02502
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 27010:Transport phenomena and unit operations-related
• Research Institution: Osaka Prefecture University
• Principal Investigator: Nomura Toshiyuki 大阪府立大学, 工学(系)研究科(研究院), 教授 (00285305)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: バイオフィルム / 原子間力顕微鏡 / ノンケミカル洗浄 / マイクロバブル / 付着力

Outline of Final Research Achievements

In this study, we investigated the suppression of biofilm formation using microbubbles (MB), which is a low environmental load type material. As a result, it was demonstrated that the cleaning efficiency was dramatically improved by optimizing the contact time between the cell and MB. It was also clarified that the bacterial cells adhered on the substrate can be removed more effectively when the MB dispersion is made acidic. In addition, it was also found that coating the surface of the substrate with nanoparticles can suppress the adhesion of bacterial cells to the substrate.

Free Research Field

細胞間に働く付着力の計測と評価

Academic Significance and Societal Importance of the Research Achievements

原子間力顕微鏡を用いた菌体に働く付着力の直接測定により、MB－菌体間付着力がMB－基板間付着力より大きくなると、MB洗浄により基板に付着した菌体を除去できること、基板表面をナノ粒子でコーティングすると菌体－基板間の付着力が低下して菌体付着を抑制できることを学術的に明らかにした。これらの結果は、MB分散液を装置に流すことによって装置を解体することなくバイオフィルム形成を抑制できる可能性を示唆している。