Construction of high-yield substance-producing microorganisms by using different sugars

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02526
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Kobe University
• Principal Investigator: Tsutomu Tanaka 神戸大学, 工学研究科, 准教授 (90436551)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 代謝工学 / バイオリファイナリー

Outline of Final Research Achievements

In this study, we developed a new metabolic engineering technology called PMPE (Parallel Metabolic Pathway Engineering). We used glucose and xylose as carbon sources for the production of target substances and cell growth, respectively, and designed the system so that the carbon derived from each sugar does not intersect within the metabolic system. After further examination of the culture medium and optimization of pH, MA was produced at 4.26 g/L after 80 hours of incubation, and the MA yield relative to consumed glucose reached 0.31 g/g after 80 hours of incubation. This yield was the highest in the world for MA production in batch operation, indicating that PMPE is an effective strategy.

Free Research Field

生物化学工学

Academic Significance and Societal Importance of the Research Achievements

低炭素社会の実現には、いかに炭素を効率よく使うか、つまり収率の向上が大きな鍵となる。しかし、微生物は投入した炭素源を物質生産ではなく菌体増殖の方へと流してしまう。そこで本研究では、Parallel Metabolic Pathway Engineering （PMPE）という新しい手法を開発した。本技術は、収率の向上のみならず、使える炭素源の種類を拡張させる点でも低炭素化に大きく貢献している。