Basic research for solving the spatio-temporal scale-gap problem in molecular recognition processes of biomolecules

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H02565
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28040:Nanobioscience-related
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Hayashi Tomohiro 東京工業大学, 物質理工学院, 准教授 (30401574)
• Co-Investigator(Kenkyū-buntansha): 石田 忠 東京工業大学, 工学院, 准教授 (80517607)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 分子ダイナミクス

Outline of Final Research Achievements

This study aimed to develop a new theoretical framework for the microscopic and macroscopic molecular binding dymics and kinetics of biomolecules. The main goal was to establish a unified reaction kinetics that can explain the interaction between microscopic binding dynamics at the single molecule level and macroscopic binding kinetics at the molecular population level. To achieve this goal, we first measured microscopic single-molecule binding interaction forces with high temporal resolution while analyzing macroscopic molecular adsorption kinetics. With this approach, we comprehensively investigated molecular binding dynamics and reaction kinetics from nanoseconds to time scales.
Furthermore, these phenomena were analyzed under various environmental conditions such as temperature, salinity, pH, and molecular adulteration. This enabled us to create a database revealing the linkages between reaction dynamics and kinetics at microscopic and macroscopic levels.

Free Research Field

ナノバイオサイエンス

Academic Significance and Societal Importance of the Research Achievements

この研究は、生物学的センシング、医学、薬学、環境科学などで大きな貢献を果たす。
まず、バイオセンシングの分野において、巨視的な反応から微視的な結合ダイナミクスを解明する能ことで、バイオセンサーの設計を大幅に改善し、その精度と信頼性を向上させます。次に、医学や薬学の分野では、体内環境での分子レベルの相互作用をシミュレートする制度が向上する。また、環境科学や産業用途においては、微視的および巨視的な相互作用の理解が改善されることで、水質検査、食品安全検査、化学物質の検出などの用途におけるバイオセンサーの効率を向上させることができる。