Development of comprehensive single-cell analysis using integrated nano-channels

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02574
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28050:Nano/micro-systems-related
• Research Institution: Nagoya University
• Principal Investigator: Arima Akihide 名古屋大学, 未来社会創造機構, 特任講師 (20781347)
• Co-Investigator(Kenkyū-buntansha): 筒井 真楠 大阪大学, 産業科学研究所, 准教授 (50546596)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: マイクロポア / イオン電流 / 1細胞解析

Outline of Final Research Achievements

In this study, it was demonstrated that cellular condition can be evaluated at a single-cell level through combining electrical trapping of a cell into an ultra-thin micropore and cross-membrane ionic current measurement. In addition, we found that this current decreasing due to the trapping was relaxed by ion transports through ion channels of a cell. Moreover, single-cell breakage was achieved by electrical dragging to a pore modified with functional polymers.

Free Research Field

マイクロ・ナノ科学

Academic Significance and Societal Importance of the Research Achievements

本研究により、単純な細孔への電気的捕捉によって1細胞の状態が非破壊・非染色で簡便に評価できることが明らかになった。また細胞破砕能を付与したポアによる1細胞レベルの破砕技術は内包粒子の抽出へと繋がるため、今後ポア集積構造へと発展させることで、内包粒子の解析に基づく網羅的な評価が可能なシステムとして機能すると考えられる。