Development of Near-Infrared Enhanced Raman Nanoprobe Method and Application to Analysis of Mechanism; External Field Excitation Cancer Cell Necrosis

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02591
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 29020:Thin film/surface and interfacial physical properties-related
• Research Institution: University of Tsukuba
• Principal Investigator: Taninaka Atsushi 筑波大学, 数理物質系, 客員准教授 (80400638)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: ナノ顕微技術 / 生体計測 / ラマン分光 / ナノプローブ / ガン細胞の特性

Outline of Final Research Achievements

We developed Single-beam induced raman spectroscopy, and a significant increase in peak intensity was observed in glutathione molecules dropped on a glass pipette deposited gold nanoparticles with a built-in liquid crystal polarizer. This demonstrated the feasibility of tip enhanced Near-Infrared raman spectroscopy. Then, we developed a multiplex coherent anti-Stokes raman scattering microscope in combination with a supercontinium laser, which is effective for low wavenumber region. Spatial variation of a sulfur crystal phase transition with metastable states was visualized.
Local elastic moduli measurements using atomic force microscopy were performed on cancer cells treated with photodynamic therapy, and the increase in elastic moduli allowed observation of actin filaments and intracellular mechanisms.

Free Research Field

表面化学

Academic Significance and Societal Importance of the Research Achievements

開発した近赤外増強ラマンナノプローブ法をさらに発展させれば、生体内において低波数振動を持つタンパク質や高分子のミクロ特性評価を進めることが可能になり、蛍光観察を行うことで細胞内基質にダメージを与えるような場合や活性酸素が蛍光プローブを失活させてしまうような実験に対して最適な手法の一つであり、アクチンフィラメントの定量や、測定が難しい細胞中の活性酸素量、および細胞が受けているストレスを弾性率の増加から見積もることが可能である。今後の展開において重要な役割を担うことが期待される。