Cryogenic single-molecule detection of hydrogen bond network in protein

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H02672
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 32010:Fundamental physical chemistry-related
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Matsushita Michio 東京工業大学, 理学院, 准教授 (80260032)
• Co-Investigator(Kenkyū-buntansha): 伊関 峰生 東邦大学, 薬学部, 教授 (60414009)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: １分子分光 / タンパク質 / 水素結合

Outline of Final Research Achievements

The aim of the present project is "Research of hydrogen bond network in protein with cryogenic single-molecule fluorescence spectroscopy". As the achievement of our project, we experimentally obtained the hydrogen bond network nearby the pigment in protein-pigment complex with the cryogenic single-molecule fluorescence spectroscopy. Despite the fact that this protein complex has thousands of CO bonds, the CO bond at hydrogen bond of the pigment binding site were highlighted.

Free Research Field

物理化学

Academic Significance and Societal Importance of the Research Achievements

本研究で得られた実験法は、１個のタンパク質中の活性な場所である色素結合サイトの水素結合状態を見ることができる方法であり、物理学、物理化学的に大きな意義のある方法である。これを、生体検査などにも応用すれば、社会的に重要な手法になると考えている。