2021 Fiscal Year Final Research Report
Ultra-sensitive cancer diagnosis based on cyclic ferrocenylnaphthalene diimide
| Project/Area Number |
19H02748
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | フェロセン化ナフタレンジイミド / 環状ナフタレンジイミド / テロメラーゼ / 電気化学的検出 / DNAチップ / 4本鎖DNA |
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| Outline of Final Research Achievements |
Telomerase is a promising marker for cancer. An electrochemical telomerase assay (ECTA) using ferrocenylnaphthalene diimides has been developed. We have previously been developing the strong binder for G-quartet DNA using novel type of cyclic naphthalenediimide. Therefore, cyclic ferrocenylnaphthalene diimides (cFNDs) 1 and 2 were developed as indicators for ECTA. cFNDs by themselves suppress the current due to the interaction of ferrocene and naphthalene diimide parts, and their binding to G-quadruplex DNA are expected to recover their expressed current. Both 1 and 2 strongly interact with TA-core, as shown derived from ITC and Tm measurements. cFND solutions were used to measure the SWV of G-quartet and single-stranded DNA-immobilized electrodes. The results showed that 2 exhibited high G-quadrupex selectivity. Finally, ECTA using 2 showed the current increase of 86% in the presence of telomerase of sample.
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| Free Research Field |
バイオ分析化学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでにフェロセン化ナフタレンジイミドによるECTAでは臨床サンプルで高精度ながんの診断を確立してきた。しかし、前がん病変の応答はがんと健常者の間に位置していた。前がんをより高精度に検出できれば、がんの超早期診断につながり、また本手法によるがんの予後診断にも利用できると思われる。がんの診断は、内視鏡検査、CTなど患者の負担の大きな検査が多い。これに代わる簡便な定量的診断方法の確立は、再発を含めたがんの早期診断につながり、患者のQOLの向上および医療費削減につながるものである。
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