2021 Fiscal Year Final Research Report
Single-cell thermometry using highly sensitive programmable temperature sensors
Project/Area Number |
19H02750
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Basic Section 34020:Analytical chemistry-related
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Research Institution | Kanazawa University |
Principal Investigator |
Arai Satoshi 金沢大学, ナノ生命科学研究所, 准教授 (70454056)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | 蛍光温度センサー / 熱産生 / 蛍光寿命 / 蛍光タンパク質 / 色素 |
Outline of Final Research Achievements |
To achieve more accurate thermometry in a live cell, we developed fluorescent sensors that allow the quantitative analysis of temperature with high sensitivity. To achieve this, the fluorescence lifetime was introduced as a robust physical parameter for the readout of temperature with the fluorescent sensor. Using a series of fluorescent sensors, we succeeded in the visualization of temperature gradient occurring at microscale using fluorescence lifetime microscopy. We further demonstrated this in the detection of temperature increment in brown adipocyte. In particular, we could obtain the insight regarding the heat transfer in the thermogenesis through the thermometry in several organelles.
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Free Research Field |
ケミカルバイオロジー
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Academic Significance and Societal Importance of the Research Achievements |
今までの生物学は、「温度」を動物個体の体温や、細胞培養の培地の温度として取り扱うことが多く、細胞1個、小器官1個の温度という視点は見落とされてきた。顕微鏡下で使用できる定量性・感度が高い細胞温度計測用のセンサーは、温度に関わる基礎生物学の分野に新しい知見をもたらす。更に、熱を積極的に用いる温熱療法(がんや筋肉)分野などに、精密温度測定技術が活かされる。
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