In-cell syntheisi of protein crystal materials

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H02830
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Ueno Takafumi 東京工業大学, 生命理工学院, 教授 (70332179)
• Co-Investigator(Kenkyū-buntansha): 古田 忠臣 東京工業大学, 生命理工学院, 助教 (10431834) ; 山下 恵太郎 東京大学, 大学院理学系研究科(理学部), 客員共同研究員 (20721690)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: タンパク質結晶 / タンパク質集合体

Outline of Final Research Achievements

Understanding the crystallization mechanism is essential to identifying the interactions between the protein monomers that make up the crystals. Based on a comparison of crystal structures of different species of polyhedra, the applicant considered that some regions are involved in the internalization of polyhedral viruses but not in the interactions that are essential for crystallization. Polyhedra crystals were genetically engineered with deletions ranging from 8 to 50 residues per monomer.

Free Research Field

生体関連化学

Academic Significance and Societal Importance of the Research Achievements

細胞を反応容器とした目的タンパク質の自在な結晶化手法を確立することができれば、有用タンパク質材料の機能化・合成・構造決定を細胞内のみで一貫して達成することが可能となり、基礎研究から社会実装まで、医薬品を中心とするバイオ分野に大きな波及効果を及ぼす技術につながる。