2022 Fiscal Year Final Research Report
Screening of novel post-translationally modified peptide pheromone
| Project/Area Number |
19H02842
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 37020:Chemistry and chemical methodology of biomolecules-related
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| Research Institution | Kanagawa University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
澄本 慎平 神奈川大学, 工学部, 助教 (20852502)
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| Project Period (FY) |
2019-04-01 – 2023-03-31
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| Keywords | 翻訳後修飾 / トリプトファン / クオラムセンシング / プレニル化 / 生物活性物質 |
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| Outline of Final Research Achievements |
Although post-translational prenylation of tryptophan residue had been identified only in Bacillus subtilis, it was found to be a universal pattern of post-translational modification widely observed in various bacteria, including Actinomycetes, Cyanobacteria, Chloroflexi, and Clostridia. Furthermore, post-translational prenylation enzyme of histidine residue has been identified from Cyanobacteria. It shows extremely high novelty because there is no report for post-translational modification of histidine residue at all.
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| Free Research Field |
天然物化学
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| Academic Significance and Societal Importance of the Research Achievements |
これまでに枯草菌のみにしか確認されていなかった、翻訳後修飾によるトリプトファン残基のプレニル化が、放線菌やシアノバクテリアなどの様々なバクテリアに広く存在する普遍的な翻訳後修飾の様式であることが判明した。さらに、トリプトファン残基ではなくヒスチジン残基のプレニル化酵素をシアノバクテリアから発見した。他の修飾様式を含めたヒスチジン残基の修飾酵素の報告例は全くなく、新規性の非常に高い翻訳後修飾酵素であった。
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