2022 Fiscal Year Final Research Report
Study of candidate proteins for a long-distance signal that transmits elevated CO2 environments and engineering of the proteins for improved CO2 responses
| Project/Area Number |
19H02857
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Review Section |
Basic Section 38010:Plant nutrition and soil science-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
金 容賢 東北大学, 農学研究科, 助教 (00871188)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 高CO2応答 / 葉の発達段階 / シグナル伝達 / イネ |
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| Outline of Final Research Achievements |
We investigated if candidate proteins for a long-distance signal that transmits elevated CO2 environments could act as the signal in rice plants. We found that the proteins were secreted extracellularly to move to tissues/organs distant from the site of synthesis, and that they likely moved from leaf blades to a basal part including developing leaves and the shoot apex in rice plants. Although we expected the candidate proteins to function in coordination with a metabolite, the level of the metabolite was not increased by elevated CO2 treatment. Thus, our hypothesis has not yet been proven. On the other hand, overexpression of the candidate protein in rice leaves did not cause phenomena occurring under elevated CO2, an indication that the candidate protein alone could not act as the signal. This observation implies that modification of the protein might alter responses to elevated CO2, leaving the growth of rice plants unaffected.
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| Free Research Field |
植物生理学、植物栄養学
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| Academic Significance and Societal Importance of the Research Achievements |
2002年に高CO2環境を伝達する長距離シグナルの存在が提唱されたが、未だシグナルの実体は明らかにされていない。我々は、イネを材料に、高CO2応答の初発反応の解析系を世界に先駆けて確立した。この解析系を用い、シグナル候補とされていた物質（e.g.,高CO2環境下で過剰産生される炭水化物、植物ホルモン）は直接的なシグナルではないことを示し、本研究課題で提唱した仮説にたどり着いた。残念ながら仮説の実証には至らなかったが、本研究課題で作製した候補タンパク質過剰発現イネを用いることによって、シグナルの実体の解明が大きく進み、変異導入によるイネの高CO2応答の改変に結びつくものと期待される。
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