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2021 Fiscal Year Final Research Report

Analysis of immunological properties of mannose-based molecules and its action mechanisms.

Research Project

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Project/Area Number 19H02902
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionTohoku University

Principal Investigator

Toda Masako  東北大学, 農学研究科, 教授 (10828429)

Co-Investigator(Kenkyū-buntansha) 佐分利 亘  北海道大学, 農学研究院, 准教授 (00598089)
比能 洋  北海道大学, 先端生命科学研究院, 教授 (70333333)
新谷 尚弘  東北大学, 農学研究科, 教授 (70374973)
Project Period (FY) 2019-04-01 – 2022-03-31
Keywordsmannose / mannan / dendritic cells
Outline of Final Research Achievements

The aim of this study was to identify immunological function of mannose-based molecules and its action mechanisms. Among α-mannooligosaccharides, long chain molecules such as α-Man-(1→6)-Man4 inhibited lipopolysaccharide (LPS)-stimulated activation of bone marrow derived murine dendritic cells (BMDC). In contrast, among β-mannooligosaccharides, β-Man-(1→4)-Man activated BMDC via engagement of toll-like receptor 4. We also found that α-mannan from yeast promotes anti-inflammatory responses (e.g., IL-10 production) in BMDC when it was stimulated with LPS. Metabolome analysis showed that α-mannan altered levels of many metabolites in BMDC, e.g., an increased level of lactic acid, the final product of glycolysis, and a reduced level of succinic acid, an inflammatory metabolite of TCA cycle. The results suggest that α-mannan induces reprogram of cellular metabolism and thereby promotes anti-inflammatory response in BMDC.

Free Research Field

食品免疫学

Academic Significance and Societal Importance of the Research Achievements

食品や微生物にはマンノ-ス骨格を持つ分子が含まれており、健康増進への活用が期待されている。本研究では、各種マンノース骨格を持つ分子の免疫機能性やその機能性発現機序を明らかにした。β型マンノオリゴ糖の中ではβ-1,4-マンノビオースが樹状細胞に対して賦活化能を持ち、一方、酵母由来αマンナンは抗炎症性を誘導することを示した。特に、酵母由来αマンナンによる樹状細胞メタボローム変化についての知見は、細胞代謝やエピジェネティックな遺伝子発現を介した樹状細胞応答のさらなる新規機序の解明、そして免疫制御のストラテジー確立に結びつく。また、本研究は科学的根拠に基づく免疫機能食品の開発にも貢献すると考えられる。

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Published: 2023-01-30  

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