Characterization of conventional microbial culture methods and development of methods for isolating and culturing of uncultured microbes

2021 Fiscal Year Final Research Report

• Project/Area Number: 19H03086
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 41050:Environmental agriculture-related
• Research Institution: University of Tsukuba
• Principal Investigator: Aoyagi Hideki 筑波大学, 生命環境系, 教授 (00251025)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 微生物培養法 / 寒天平板培地 / フラスコ振盪培養 / ダークマター微生物 / 未培養微生物

Outline of Final Research Achievements

The characteristics of representative culture methods for microbes (shake flask culture method and the agar plate culture method) were analyzed. The behavior of the gas and liquid phases during shake flask culture of microbes were analyzed by using originally constructed systems. The cultured microbes were affected by various factors associated with the sampling operation. The analysis of the agar plate culture method elucidated the changes in various factors and characteristics that occur in the plate culture medium, and the problems associated with the conventional method were clarified.

Free Research Field

生物化学工学、細胞機能開発工学、応用微生物学、細胞培養工学

Academic Significance and Societal Importance of the Research Achievements

自然界に存在する微生物の1%程度しか培養できない”という課題は、様々な微生物関連分野で共通の問題である。本研究で得られた成果は、従来の微生物培養法の特性を解析、把握することで得られる新たな知見をベースに、多様な未培養微生物の培養化に貢献できる方法論を提案しており国内外の大学、研究機関や微生物関連産業で広く使用でき、学術・産業面のイノベーション創出に貢献できる。